双氢青蒿素对前列腺癌裸鼠种植瘤Bcl-2和Bax表达的调控  

Regulatory effects of dihydroartemisinin on Bcl-2 and Bax expression in implanted prostate cancer in nude mice

作　　者：李庆春[1,2] 高小玲[1] 邹聪[1] 罗子国[1] 李剑[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016 [2]重庆西南铝医院,重庆401326

出　　处：《第二军医大学学报》2010年第1期24-27,共4页Academic Journal of Second Military Medical University

摘　　要：目的研究双氢青蒿素(DHA)诱导前列腺癌细胞凋亡作用及其机制。方法采用人前列腺癌PC-3细胞株建立裸鼠种植瘤模型,将20只模型鼠随机平均分为对照组、溶剂(DMSO)组、DHA200μmol/kg体质量组和DHA100μmol/kg体质量组,经13d干预后,电镜观察肿瘤组织形态学表现;免疫组化检测凋亡相关基因Bcl-2、Bax的表达水平;TUNEL法检测肿瘤组织细胞凋亡。结果与对照组及DMSO组比较,DHA治疗组电镜观察肿瘤组织中散在凋亡细胞及凋亡小体;免疫组化检测凋亡相关基因Bcl-2表达减弱、Bax表达增强,差异均有统计学意义(P<0.05);TUNEL检测结果显示肿瘤组织细胞凋亡率明显升高,差异均有统计学意义(P<0.05)。结论DHA具有较强的诱导前列腺癌细胞凋亡作用,这种作用可能是通过下调Bcl-2和上调Bax表达实现的。Objective To investigate dihydroartemisinin（ DHA）-induced apoptosis of human prostate cancer cells and the underlying molecular mechanism. Methods Nude mice were implanted with human prostate cancer PC-3 cells to establish tumor-bearing mouse model. Twenty models mice were evenly randomized into four groups：control group,solvent group（ DMSO） ,large dose DHA （ 200 μmol/kg） group and low dose DHA （ 100 μmol/kg） group. The implanted tumors were observed on day 13 after drug administration. Morphological changes of PC-3 cells were observed by transmission electron microscope（ TEM）. The protein expression of apoptosis associated gene Bcl-2 and Bax were examined by immunohistochemical method. Apoptosis was detected by TUNEL assay. Results TEM examination revealed scattered apoptotic cells and apoptotic bodies in tumor tissues of mice in the DHA groups; immunohistochemical examination revealed that the protein expression of apoptosis-associated gene Bcl-2 was decreased and that of Bax was increased in DHA groups（ P 〈 0. 05）. TUNEL staining revealed that the rate of cell apoptosis increased significantly in DHA groups（ P 〈 0. 05）. Conclusion It is demonstrated that DHA has strong effect in inducing apoptosis in human prostate cancer cell line PC-3 in vivo,which might be related to the down -regulation of Bcl-2 and up-regulation of Bax.

关 键 词：双氢肯蒿素 前列腺肿僖 细胞凋亡 BCL -2 BAX 

分 类 号：R737.25[医药卫生—肿瘤]