喉癌细胞中表皮钙粘蛋白、Ⅳ型胶原表达与颈淋巴结转移的关系  

The relationship of expression of epithelial-cadherin and type Ⅳ collagen in larynx carcinoma with jugular lymph node metastasis

作　　者：胡春英[1] 任重[2] 

机构地区：[1]沈阳市第七医院耳鼻喉科,沈阳110003 [2]中国医科大学附属第一医院耳鼻咽喉科,沈阳110001

出　　处：《解剖科学进展》2010年第1期51-53,共3页Progress of Anatomical Sciences

摘　　要：目的研究癌转移抑制基因钙粘蛋白(E-CD)和基底膜(BM)的主要成分Ⅳ胶原蛋白(ColIV)在喉癌中的表达与颈淋巴结转移的关系。方法采用免疫组织化学方法(SABC)检测E-CD和ColIV在喉癌中的表达。按结果判定标准,进行统计学分析。结果声门上型喉癌中E-CD阳性表达率为58.82%(30/51),在喉癌Ⅰ-Ⅱ期占90.90%(10/11),Ⅲ-Ⅳ期占50.00%(20/40),二者差异显著(P<0.05)。E-CD阳性表达率在喉癌颈淋巴结转移组(34.48%,10/29)明显低于非转移组(90.90%,20/22,P<0.01)。ColIV在喉癌阳性表达率为60.78%,Ⅰ-Ⅱ期为100.00%(11/11),Ⅲ-Ⅳ期为50.00%(20/40),二者差异显著(P<0.05)。ColIV阳性表达在颈淋巴结转移组37.93%(11/29)明显低于非转移组为90.90%(20/22,P<0.01)。结论喉癌组织中E-CD及ColIV的表达呈正相关,与颈淋巴结转移无明显相关性。Objective To study the relationship of the expression of epithelial-cadherin（E-CD） and type Ⅳ collagen（ColIV）in the larynx carcinoma with the jugular lymph node metastasis.Methods The immunohistological chemical method（SABC method）was used to ditect the expression of E-CD and ColⅣin the larynx carcinoma.Results In the 51 cases of superglottic larynx carcinoma,the total positive rate of E-CD expression was 58.82%（30/51）,among which was 90.90%（10/11）in phaseⅠand Ⅱ of larynx carcinoma and 50.00%（20/40）in phase Ⅲ and Ⅳ（p〈0.05） respectively.The E-CD positive expression rate was higher in nonmetastasis of jugular lymph node（90.90%,20/22）than in metastasis of jugular lymph node（34.48%,11/29,p〈0.01）.Moreover,the total positive rate of ColⅣ was 60.78%（31/51）,among which was higher in phaseⅠandⅡ of larynx carcinoma（100.00%,11/11）than in phase Ⅲand Ⅳ（50.00%,20/40,p〈 0.05） .The expression rate of ColⅣ was higher in nonmetastasis of jugular lymph node（90.90%,20/22）than in metastasis of jugular lymph node（37.93%,11/29,pp〈0.01）.Conclusions The expression of E-CD and ColⅣ in larynx carcinoma was correlated positively,but not related to the jugular lymph node metastasis.

关 键 词：喉肿瘤 表皮钙粘蛋白 Ⅳ型胶原 颈部淋巴结转移 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]