Prohibitin在单侧输尿管梗阻大鼠肾间质纤维化中的表达和作用  

Expression and effects of Prohibitin in unilateral ureteral obstruction rats with renal interstitial fibrosis

作　　者：赵艳君[1] 覃远汉[1] 陈静[1] 雷凤英[1] 胡鹏[2] 庞玉生[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021 [2]安徽医科大学第一附属医院儿科,安徽合肥230022

出　　处：《临床儿科杂志》2010年第1期69-72,共4页Journal of Clinical Pediatrics

基　　金：广西自然科学基金资助项目(No.桂科自0832121)

摘　　要：目的探讨Prohibitin(PHB)在单侧输尿管梗阻(UUO)大鼠肾脏组织中的表达和作用。方法48只雄性6周龄Wistar大鼠随机分为假手术组和模型组,每组24只,模型组大鼠麻醉后,背侧切口行左侧输尿管结扎术,假手术组只探及肾包膜,造模后分别于第7、14、21、28天各处死6只大鼠,行肾脏病理检查并计算肾间质纤维化指数,采用实时荧光定量PCR和免疫组织化学方法检测肾脏组织中PHB与转化生长因子-β1(TGF-β1)mRNA和蛋白表达。结果与假手术组相比,模型组各时间点肾间质纤维化指数显著增高(P<0.01),梗阻时间越长纤维化指数越高;模型组各时间点PHB的mRNA和蛋白表达显著减少(P<0.01),梗阻时间越长,表达量越低;模型组各时间点TGF-β1的mRNA和蛋白的表达显著增高(P<0.01),梗阻时间越长,表达量越高。模型组PHB的蛋白表达与间质纤维化指数呈显著负相关(r=-0.825),TGF-β1的蛋白表达与间质纤维化指数呈显著正相关(r=0.995),PHB蛋白表达与TGF-β1蛋白表达呈显著负相关(r=-0.786)。结论UUO大鼠肾脏组织PHB表达显著降低,可能参与肾间质纤维化的发生发展。Objective To detect and investigate the expression and the effect of Prohibitin （PHB） in rats with renal interstitial fibrosis （RIF） induced by unilateral ureteral obstruction （UUO） . Methods Forty-eight Wistar male rats （6-weeks-old） were randomly assigned into 2 groups,sham-operated and model group. The model group rats were subjected to left ureteral ligation after anesthesia and the sham-operated group rats were subjected to sham operation. Six rats were killed 7,14,21,28 days after operation respectively. The renal tissues were collected. The index of RIF was calculated. The expressions of mRNA and protein of PHB were assayed by real time polymerase chain reaction and immunohistochemistry. Results Compared with sham-operation group,at each time point,the model group had significantly increased index of RIF （P 〈 0.01） and the obstruction for a longer period showed the higher index; the model group had significantly decreased expression of mRNA and protein of PHB （P 〈 0.01） and the obstruction for a longer period showed the lower expression; the model group had significantly increased expression of mRNA and protein of TGF-β1 （ P 〈 0.01） and the obstruction for a longer period showed the higher expression. Correlation analysis showed that the index of RIF was negatively correlated with PHB （r = -0.825） and positively correlated with TGF-β1 （r = 0.995）,while there was a positive correlation between PHB and TGF-β1 （r = -0.786）. Conclusions The lower expression of PHB in renal tissue of UUO rats might suggest that it play an important role in RIF.

关 键 词：PROHIBITIN 肾间质纤维化 转化生长因子-Β1 

分 类 号：R693.2[医药卫生—泌尿科学]