帕金森病模型大鼠中脑α-突触核蛋白增加  

Increased protein level of α-synuclein in the midbrain of Parkinson's disease rat model

作　　者：孙建栋[1] 苑玉和[1] 刘岩[1] 王晓锋[1] 陈乃宏[1] 

机构地区：[1]中国医学科学院药物研究所,北京协和医学院药物研究所,北京100050

出　　处：《中国药理学通报》2010年第1期73-77,共5页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)子课题(No2004CB518906);国家自然科学基金资助项目(No30973887);国家自然科学基金广东自然科学联合基金资助项目(NoU0832008);长江学者和创新团队发展计划(PCSIRTNoIRT0514);中央级公益性科研院所基本科研专项计划-青年基金(No2007CHX04);创新药物研究开发技术平台建设(No2009ZX09303);创新药物研究开发技术平台建设(No2009ZX09301)

摘　　要：目的观察鱼藤酮对大鼠中脑α-突触核蛋白(α-SYN)的影响。方法SD大鼠长期低剂量皮下注射1.0mg·kg-1·d-1的鱼藤酮,建立帕金森病(PD)动物模型。采用旷场实验评价动物行为学改变,观察大鼠5min的活动情况。免疫荧光化学方法检测中脑部位TH阳性神经元数目的变化。免疫组织化学和Western blot方法检测中脑部位α-SYN的蛋白水平的改变。结果同对照组相比,鱼藤酮实验组动物5min的正中格停留时间明显延长(P<0.05),方格穿行次数明显减少(P<0.05),竖起时间和次数明显减少(P<0.01);中脑部位TH阳性神经元数目明显减少(P<0.05),α-SYN蛋白水平明显增加(P<0.05)。结论给予鱼藤酮能够使SD大鼠出现运动障碍,中脑部位TH阳性神经元数目减少,该现象可能与大鼠中脑部位α-SYN蛋白水平增加有关。Aim To study the effect of rotenone on α-synuclein in rat midbrain of Parkinson＇s disease（PD）. Methods Rats were subcutaneouly injected with chronic low dose rotenone （1.0 mg·kg-1·d-1 ）. Movements within 5 minutes were evaluated in open field test. Tyrosine hydroxylase（TH）-positive neurons in the midbrain were measured with immunofluores-cence staining. α-SYN protein level in the midbrain was demonstrated with immunohistochemical staining and Western blot. Results Compared to the control group,latency time,crossing,rearing and rearing time were changed significantly（P〈0. 05,0. 01）in the rotenone group,TH-positive neurons were reduced （P〈0.05）and α-SYN protein level in the midbrain was increased（P〈0.05）. Conclusion Injection with rote-none can induce PD symptom,which may be correlated to α-SYN protein level in the midbrain.

关 键 词：帕金森病 鱼藤酮 Α-突触核蛋白 酪氨酸羟化酶 旷场实验 中脑 

分 类 号：R-332[医药卫生] R322.81