环氧合酶-2选择性抑制剂NS-398诱导人肝癌BEL-7402细胞凋亡及其机制探讨  

Effects of selective cyclooxygenase-2 inhibitor NS-398 on apoptosis and survivin,XIAP and c-IAP1 expressions of hepatocellular carcinoma BEL-7402 cells

作　　者：付卫争[1] 孙国平[1] 范璐璐[1] 葛磊[1] 吴志丽[1] 

机构地区：[1]安徽医科大学第一附属医院肿瘤内科,合肥230022

出　　处：《肿瘤》2010年第1期11-14,共4页Tumor

基　　金：国家自然科学基金资助项目(编号:30772537)

摘　　要：目的:探讨环氧合酶-2(cyclooxygenase-2,COX-2)选择性抑制剂NS-398对人肝癌BEL-7402细胞凋亡及凋亡抑制蛋白survivin、XIAP和c-IAP1表达的调节作用。方法:用不同浓度的NS-398作用BEL-7402细胞后,MTT法测定细胞增殖抑制情况,FCM法和TUNEL法检测细胞凋亡情况,免疫细胞化学法检测COX-2、survivin、XIAP和c-IAP1蛋白的表达情况。结果:NS-398可以显著抑制BEL-7402细胞的增殖,诱导其凋亡。免疫细胞化学法检测结果显示,与未处理组相比,NS-398作用可使BEL-7402细胞中COX-2、survivin、XIAP和c-IAP1蛋白的表达明显下调(P<0.01)。结论:NS-398对人肝癌细胞株BEL-7402有抑制细胞增殖和诱导细胞凋亡的作用,其机制可能与通过下调survivin、XIAP和c-IAP1的表达有关。Objective：To investigate the effect and elucidate the mechanism of the selective cyclooxygenase-2（COX-2）inhibitor NS-398 on apoptosis and survivin,XIAP and c-IAP1 expressions of hepatocarcinoma cell lines.Methods：The proliferation of hepatocarcinoma BEL-7402 cells treated with NS-298 at different concentrations were evaluated by MTT assay.The apoptosis was detected by flow cytometry（FCM） and TUNEL assay.Expressions of COX-2,survivin,XIAP and c-IAP1 were analyzed by immunocytochemical staining.Results： NS-398 significantly inhibited cell proliferation of BEL-7402 cells and induced apoptosis.Immunocytochemisty indicated that the expressions of COX-2,survivin,XIAP and c-IAP1 were significantly down-regulated in BEL-7402 cells by NS-398 treatment compared with untreatment group（P0.01）.Conclusion：NS-398 inhibits the proliferation and induced apoptosis of BEL-7402 cells.The mechanism may be related with down-regulation of the survivin,XIAP and c-IAP1 expression.

关 键 词：癌 肝细胞 环氧合酶2抑制剂 细胞凋亡 凋亡抑制蛋白质类 NS-398 

分 类 号：R735.7[医药卫生—肿瘤]