外源一氧化氮对胃癌SGC-7901细胞增殖及细胞周期的影响  

Effects of exogenous NO on cell proliferation and cell cycle of gastric cancer cell line SGC-7901

作　　者：桑建荣[1] 陈永昌[1] 邵根宝[2] 黄晓佳[3] 

机构地区：[1]江苏大学基础医学与医学技术学院生理学系,镇江212013 [2]江苏大学基础医学与医学技术学院生物学系,镇江212013 [3]江苏大学基础医学与医学技术学院药理学系,镇江212013

出　　处：《肿瘤》2010年第1期21-25,共5页Tumor

基　　金：江苏大学高级专业人才科研启动基金资助项目(编号:08JDG033)

摘　　要：目的:探讨外源一氧化氮(nitric oxide,NO)对胃癌细胞生长和增殖的影响。方法:应用MTT法评价NO供体硝普钠(sodium nitroprusside,SNP)和一氧化氮合酶(nitric oxide synthase,NOS)抑制剂N-硝基-L-精氨酸甲酯(N-nitro-L-argininemethylester,L-NAME)对胃癌SGC-7901细胞生长的抑制作用,RT-PCR和Western印迹法检测增殖细胞核抗原(proliferating cellnuclear antigen,PCNA)和Caspase-3 mRNA和蛋白的表达,FCM法检测细胞周期的变化。结果:SNP使细胞周期发生变化,与对照组比较,S期细胞减少,G0/G1期细胞增多,细胞由G0/G1期进入S期延缓;SNP抑制SGC-7901细胞的生长,PCNA mRNA、PCNA蛋白和Caspase-3蛋白表达降低。而NOS抑制剂L-NAME预处理逆转了SNP的这种作用。结论:NO可抑制胃癌细胞生长和增殖,加速细胞凋亡。Objective：To investigate the effect of exogenous nitric oxide（NO） on the growth and proliferation of gastric cancer cell line SGC-7901.Methods：The inhibitory effects of NO donor sodium nitroprusside（SNP） and nitric oxide synthase（NOS） inhibitor N-nitro-L-arginine methylester（L-NAME） on the proliferation of SGC-7901 cells were analyzed by MTT assay.The changes of mRNA and protein expression of proliferating cell nuclear antigen（PCNA） and caspase-3 were examined by RT-PCR and Western blotting.The cell cycle was measured using flow cytometry.Results：Compared with control group,more cells in the SNP group were arrested at G1 and G0 phases（P0.05） and fewer cells were at S phases（P0.05）.SNP decreased the speed of cell-cycle progression from G0/G1 phase into S phase.SNP inhibited the proliferation of SGC-7901 cells and reduced the mRNA and protein expressions of PCNA and caspase-3.NOS inhibitor L-NAME reversed the effects of SNP.Conclusion：NO inhibited cell growth and proliferation,but accelerated apoptosis of gastric cancer cells.

关 键 词：胃肿瘤 一氧化氮供体 一氧化氮合酶 增殖细胞核抗原 细胞周期 

分 类 号：R735.2[医药卫生—肿瘤]