四种纳米粒径钛片生物相容性检测  

Biocompatibility of Titanium Films with Different Nano-size

作　　者：李明霞[1] 李晓敏[2] 张静[1] 王虎[1] 李伟[3] 缪竞威[4] 

机构地区：[1]四川大学华西口腔医院放射科,四川成都610041 [2]广东省口腔医院.南方医科大学附属口腔医院 [3]口腔生物医学工程教育部重点实验室 [4]四川大学原子核科学技术研究所

出　　处：《广东牙病防治》2010年第1期11-14,共4页Journal of Dental Prevention and Treatment

基　　金：国家自然科学基金资助项目(10675087)

摘　　要：目的研究4种纳米粒径钛片的生物相容性。方法分别在常温、100℃、250℃和380℃,采用直流磁控溅射法,形成4种纳米粒径表面的钛片。在24孔培养板上,将SD(Sprague-Dawley)乳鼠第3代成骨细胞接种于4种纳米粒径的钛片和未处理的钛片表面,分别为常温组、100℃组、250℃组、380℃组、非处理组;另设1个空白对照组,细胞培养板内不放入钛片。四甲基偶氮唑盐比色法测定6组细胞的增殖情况。结果培养后第1、4、7天,250℃组和380℃组材料表面细胞持续增殖,其中250℃组最高;培养后第7天,4个温度处理组细胞光密度值与非处理组的差异均有统计学意义(P<0.05)。结论纳米化纯钛材料表面可促进成骨细胞的增殖。Objective To explore the biocompatibility of implant materials after surface modification,and to test cell proliferation on the nano-scale titanium films of titanium surface. Methods According to different sputtering temperatures,four different nano-granule titanium films were prepared by direct magnetron sputtering. Rat osteoblasts were seeded on the surface of different nano-granule titanium film samples and non-treated Ti sample （control group）. Cell proliferation on titanium surface was evaluated by using [3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazoliumbromide,MTT] colorimetry. Results Cell proliferation test results in titanium surface showed that with the incubation time from1 d,4 d to 7 d,250 ℃ and 380 ℃,in the experimental group the cells showed continuous proliferation,and reached the highest value at 250 ℃. Significant differences were observed between those from experimental groups and control. （P〈0.05）. Conclusion Nanosizing titanium surface could significantlyimprove osteoblast proliferation.

关 键 词：纳米结构 钛片 成骨细胞 表面改性 细胞增殖 

分 类 号：R783.1[医药卫生—口腔医学]