延迟性和痕迹性条件反射建立过程中豚鼠眨眼反应特征参数的变化  

Characteristic parameters of eyeblink responses during acquisition of delay and trace conditioning in guinea pigs

作　　者：陈浩[1] 胡波[1] 杨丽[1] 范郑丽[1] 隋建峰[2] 

机构地区：[1]第三军医大学基础医学部:生理学教研室,重庆市神经科学研究所,重庆400038 [2]第三军医大学基础医学部:教学实验中心,重庆400038

出　　处：《第三军医大学学报》2010年第2期95-98,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30771769)~~

摘　　要：目的研究延迟性和痕迹性条件反射建立过程中豚鼠眨眼反应特征参数的变化。方法采用500 ms正弦波纯音和100 ms束状氧气流的配对刺激,训练豚鼠建立延迟性和痕迹性眨眼条件反射。在此过程中,使用张力换能器记录豚鼠左侧眼轮匝肌活动,并使用我室编制的Matlab软件分析眨眼行为数据。结果2组豚鼠均分别能建立延迟性和痕迹性眨眼条件反射。但是,相对于痕迹性条件反射训练组,延迟性条件反射训练组豚鼠表现出了更快的条件眨眼反应习得率和更高的条件眨眼反应峰幅度(P<0.01)。2组豚鼠条件眨眼反应峰潜伏期以及非条件眨眼反应的峰幅度和峰潜伏期在10 d条件反射训练过程中变化均不显著(P>0.05)。结论在2类条件反射训练过程中,豚鼠眨眼反应特征参数的变化规律与其他常用啮齿类动物模型相类似。Objective To investigate the changes of characteristic parameters in the eyeblink responses during the acquisition of delay and trace eyeblink conditioning in albino guinea pigs.Methods A 500 ms tone conditioned stimulus（CS） was paired with a 100 ms corneal oxygen-puff unconditioned stimulus（US）.Totally 12 guinea pigs were trained for 10 consecutive sessions of delay（n=6） and trace eyeblink conditioning（n=6）.During the experiments,a spring-return potentiometer was used to detect the activity of orbicularis oculi muscle.Homemade software written under the Matlab environment was used to quantitatively analyze the behavioral data.Results Both groups of guinea pigs successfully acquired the delay and trace conditioned eyeblink responses（CRs）,respectively.Relative to the trace-conditioned guinea pigs,the delay-conditioned guinea pigs acquired the CRs with faster rates and higher peak amplitudes（P0.01）.However,the CR peak latency,the peak amplitude and latency of the unconditioned eyeblink responses did not significantly change during 10 eyeblink conditioning sessions（P0.05）.Conclusion During eyeblink conditioning,the guinea pigs exhibit characteristic parameter changes of eyeblink responses similar to those observed in other common-used rodents.

关 键 词：延迟性条件反射 痕迹性条件反射 眨眼 豚鼠 

分 类 号：R-332[医药卫生] R338.61