头颈鳞状细胞癌手术切缘P53表达及意义  

Expression and Significance of P53 Protein in Surgical Margins of Patients with Head and Neck Squamous Cell Carcinoma

作　　者：李超[1] 张兵[1] 陈锦[1] 王朝晖[1] 李伦[2] 姜庆华[1] 宋宇峰[3] 

机构地区：[1]四川省肿瘤医院研究所头颈外科,成都610041 [2]四川省肿瘤医院研究所病理科,成都610041 [3]贵阳医学院附属医院口腔颌面外科

出　　处：《肿瘤防治研究》2010年第1期52-55,共4页Cancer Research on Prevention and Treatment

基　　金：四川省卫生厅科研课题资助项目(070030)

摘　　要：目的探讨头颈鳞癌手术切缘P53蛋白的表达及其与肿瘤临床病理学参数和局部复发的关系。方法用免疫组织化学的方法检测82例头颈鳞癌手术切缘标本和10例正常口腔黏膜组织中P53的表达情况,并分析其与肿瘤局部复发和临床病理参数的关系。结果在82例头颈鳞癌患者中,切缘突变型P53阳性表达率为59.76%(49/82)。阳性表达主要定位于切缘上皮基底细胞核。突变型P53蛋白在头颈鳞癌手术切缘中的表达显著高于其在正常口腔粘膜组织中的表达(P<0.05)。头颈鳞癌患者手术切缘突变型P53的表达与肿瘤分化程度和局部复发显著相关(P<0.05),但与患者年龄、性别、肿瘤病理分期、淋巴结转移及肿瘤部位无关(P>0.05)。结论切缘突变型P53的阳性表达与头颈鳞癌术后肿瘤的进展有关,它有望成为判定肿瘤局部复发的预测因子。Objective To explore the expression of P53 protein in the surgical margins of patients with head and neck squamous cell carcinoma （HNSCC）, and its relationship between clinicopathological parameters and tumor local recurrence. Methods The expression of P53 protein in 82 cases of human HNSCCs and 10 cases of normal oral mucosa was detected by using immunohistochemistry. The correlations among P53 protein to clinicopathological parameters and tumor local recurrence in these cases was investigated. Results Of the 82 cases of HNSCC, 59. 76% （49/82） in surgical margins were positive for P53 protein. P53 protein was mainly detected in nucleus of basal cell in surgical margins. The expression of P53 protein was significantly higher in surgical margins of patients with HNSCC than that in normal o- ral mucosa （P〈0. 05）. P53 protein positive expression was significantly correlated with tumor differentiation and tumor local recurrence in 82 cases of HNSCC （P〈0. 05）, but not with age and gender of patients, pathological stage, lymph node metastasis and anatomic site of tumor were not found （P〉0. 05）. Conclusion These results suggest that the presence of P53 protein in tumor surgical margins correlates with the development of HNSCC after operation. P53 protein may serve as one prognostic factor for tumor local recurrence.

关 键 词：头颈鳞状细胞癌 P53 切缘组织 局部复发 

分 类 号：R739.91[医药卫生—肿瘤]