中药抗纤灵方含药血清对TGF-β_1刺激的HK-2细胞c-Met及其下游MAPK信号分子的调控作用  

Effects of sera containing Kang xian ling on c-Met and its downstream MAPK signal molecules in HK-2 cells treated with TGF-β_1

作　　者：陆海英[1] 刘克剑[1] 张悦[1] 何立群 刘煜敏[1] 李靖[1] 韩志芬[1] 庞慧芳[1] 

机构地区：[1]上海中医药大学科技实验中心 [2]上海中医药研究院肾病研究所,上海201203

出　　处：《中国病理生理杂志》2010年第1期154-157,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30371834)

摘　　要：目的:观察中药抗纤灵方对转化生长因子β1(TGF-β1)刺激后肾小管上皮细胞(TECs)中肝细胞生长因子(HGF)受体c-Met及其下游丝裂原激活的蛋白激酶(MAPK)信号分子的调控作用,以初步探讨其延缓肾纤维化的作用机制。方法:给予大鼠中药灌胃3d后制备抗纤灵含药鼠血清,应用不同含药血清处理经TGF-β1刺激的HK-2细胞,然后用Alarmblue法和Western blotting法检测细胞增殖率、c-Met和MAPK磷酸化情况。结果:TGF-β1和含药鼠血清对HK-2细胞增殖率无明显影响,含药血清影响TGF-β1刺激的HK-2细胞c-Met的表达及MAPK的磷酸化。结论:抗纤灵含药血清可调节HGF受体c-Met介导的MAPK的磷酸化。AIM： To observe the effects of sera containing Kang xian ling （a traditional Chinese medicine） on c - Met and its downstream MAPK signal molecules in HK - 2 cells treated with transforming growth factor -β1 （ TGF -β1 ）, so as to further explore the effective mechanism of anti - renal fibrosis of the traditional Chinese medicine. METHODS ： Rat sera containing herbs were collected after rats were intragastrically administered with the herbs. HK - 2 cells activated by TGF -β1 were incubated with different heat - inactivated sera. The proliferation and expression of MAPK and the phosphorylation of MAPK in HK - 2 cells were detected by Alarm blue and immunoblotting. RESULTS ： No significant difference of cell proliferation between any two groups was observed, c - Met and the phosphorylation of its downstream molecules were also observed after incubated with different sera. CONCLUSION： The receptor c - Met of hepatocyte growth factor, and the phosphorylation of its downstream MAPK molecules can be regulated by the sera containing Kang xian ling.

关 键 词：抗纤灵 肾小管上皮细胞 转化生长因子β 肝细胞生长因子 MAPK信号通路 

分 类 号：R363[医药卫生—病理学]