肝肽、脾肽对荷瘤裸鼠肿瘤细胞中Bcl-2、Bax表达的影响  

THE EFFECTS OF ACBP-L AND ACBP-S ON EXPRESSION OF BCL-2 AND BAX IN GASTRIC CANCER-LOADED NUDE MICE

作　　者：娜仁图雅[1] 苏丽娅[2] 刘桂荣[2] 苏秀兰[2] 

机构地区：[1]锡林郭勒职业学院医学系基础教研室,内蒙古锡林郭勒026000 [2]内蒙古医学院附属医院临床医学研究中心

出　　处：《内蒙古医学院学报》2009年第6期548-551,共4页Acta Academiae Medicinae Neimongol

基　　金：国家自然科学基金(30860327)

摘　　要：目的:观察两种抗癌生物活性肽对荷瘤裸鼠肿瘤细胞中Bcl-2、Bax表达的影响。方法:建立人胃癌裸鼠模型,裸鼠随机分为三组:对照组11只、脾肽实验组12只,肝肽实验组11只;分别尾静脉注射生理盐水0.2mL、脾肽0.2mL,肝肽0.2mL,隔日给药2wk后处死裸鼠,剥取肿瘤,以4%多聚甲醛固定,常规石蜡包埋,4μm切片。应用免疫组化法检测Bcl-2、Bax蛋白在实验组与对照组中的表达情况。结果:Bcl-2蛋白在对照组肿瘤中阳性表达较强,肝肽、脾肽组裸鼠肿瘤中Bcl-2蛋白的表达均比对照组表达弱,且有显著性差异(P<0.001);Bax蛋白在对照组肿瘤中阳性表达较弱,在肝肽组、脾肽组裸鼠肿瘤中Bax蛋白的表达均比对照组的表达强,有显著性差异(P<0.001)。结论:肝肽、脾肽具有较好的抑制荷胃癌裸鼠肿瘤生长的作用,其作用机制与影响Bcl-2及Bax的表达相关。Objective：To explore the effects of the expression changes of anti - cancer bioacfive peptide - liver （ACBP - L） and anti - cancer bioactive peptide - spleen （ ACBP - S） on the expression of Bax and Bcl - 2 in gastric cancer - loaded nude mice. Methods： The nude mice xenografts model with gastric carcinoma cell were established, nude mice were divided into three groups randomly： 11 cases of nude mice as control group, 12 cases of nude mice as ACBP - L treatmend group and 11 cases of nude mice as ACBP - S treatment group. Three groups of nude mice were administrated into the tail vein of the mouse every day with 0.2mL physiologic saline, 0.2mL ACBP - L and ACBP - S respectively until two weeks. The mice were killed after one month , tumors were taken off and weighed. The tumors were decohesed and fixed with 4% paraformaldehyde, rutine paraffin embedded and sliced into 4μm. Then Bax and Bcl -2 expression were detected using immunohistochemistry SABC method between experimental and control groups. Results：The expression of Bax was relatively weaker in control group than those in experimental groups and which showed significant difference （P 〈0. 001 ）. While the expression of Bcl -2 was stronger in control group than those in experiment groups and which showed significant difference （P 〈 0. 001 ）. Conclusion： ACBP - S and ACBP - L have marked inhibiting effects upon the growth of BGC -823 cell in nude mice. The mechanism is probably related with its regulatory effects on Bcl -2 and Bax.

关 键 词：肝肽 脾肽 细胞凋亡Bcl-2 BAX 

分 类 号：R363.14[医药卫生—病理学]