角质细胞生长因子对大鼠肝癌细胞CBRH-7919增殖的影响  

The effect of keratinocyte growth factor on the proliferation of rat hepatoma cell line CBRH-7919

作　　者：李京敏[1] 秦燕霞[2] 白咸勇[1] 李雅娜[1] 

机构地区：[1]滨州医学院组织学与胚胎学教研室,烟台市264003 [2]济宁医学院解剖学教研室

出　　处：《滨州医学院学报》2009年第6期410-412,共3页Journal of Binzhou Medical University

基　　金：教育部高等学校优秀骨干教师基金项目资助

摘　　要：目的研究角质细胞生长因子(keratinocyte growth factor,KGF)对大鼠肝癌细胞CBRH-7919增殖的影响。方法MTT法检测不同浓度的重组人角质细胞生长因子(K102)对CBRH-7919细胞增殖的影响;免疫细胞化学技术检测角质细胞生长因子受体(keratinocyte growth factor receptor,KGFR)在CBRH-7919细胞中的表达。结果KGFR在CBRH-7919细胞的胞膜和胞质中均有表达,MTT实验显示K102对CBRH-7919细胞增殖作用与对照组相比无统计学差异(P>0.05)。结论KGFR在CBRH-7919细胞的胞膜和胞质中存在表达,但K102对肝癌细胞CBRH-7919的增殖无明显作用。Objective To investigate the impact of KGF on the proliferation of rat hepatoma cell line CBRH-7919.Methods MTT assay was performed to measure the influence of recombinant human keratinocyte growth factor（K102） on the proliferation of CBRH-7919.Immunocytochemistry technique was employed to determine the expression of KGFR in CBRH-7919 cells.Results KGFR was expressed in the CBRH-7919 cells.MTT experiment showed that the growth of CBRH-7919 cells was not significantly influenced by K102（P0.05）.Conclusion KGFR was positive in CBRH-7919 cells,but the proliferation of CBRH-7919 cells was not significantly influenced by K102.

关 键 词：CBRH-7919细胞 KGF KGFR 细胞增殖 

分 类 号：R73-3[医药卫生—肿瘤]