Protein A/G亲和层析在HCV抗体纯化中的应用效果  

Application Effect of Protein A/G Affinity Chromatograph in Heapatitis C Virus Antibody Purification

作　　者：杨蓉[1] 谢忠平[1] 龙润乡[1] 白惠珠[1] 谭振国[1] 王燕[1] 吴忠香[1] 张勇[1] 

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所,昆明650118

出　　处：《医学研究杂志》2010年第1期24-26,共3页Journal of Medical Research

基　　金：国家863资助项目(2007AA02Z480);云南省联合支持国家计划项目(2008GA008)

摘　　要：目的分析蛋白A/G亲和层析柱在纯化抗HCV血清的影响因素,以便选择最佳使用条件提高ProteinA/G亲和柱的利用效率。方法分别将抗HCV血清用不同处理方式、不同上样方式、不同使用次数的亲和柱进行纯化,并收集纯化样品进行检测、分析。结果用饱和硫酸铵粗纯后,在蛋白A/G亲和层析柱的纯化效果更好;样品在亲和柱内吸附30min可提高亲和柱的对目的蛋白的吸附。结论使用初纯的抗体并增加吸附时间能提高亲和层析柱的利用率,并有效延长亲和柱的使用寿命。ObjectiveTo analyze the factors of Protein A/G affinity column,influencing purification effect, and get the best condition to improve application effect of Protein A/G affinity column. MethodsWe Purified anti-HCV-IgG serum with different disposal modality, different sample input modality and different application number of affinity column before detecting and analyzing the purified samples. ResultsThe Protein A/G affinity column had the best purified effect after using saturated ammonium sulfate to first purification, which increased the affinity column adsorption effect within 30 minutes adsorption. ConclusionUsing antibody with first purification and adding the adsorption time could improve utilization rate of affinity column and prolong the service life.

关 键 词：PROTEIN A/G 亲和层析 抗体 纯化 丙型肝炎病毒 

分 类 号：R457.2[医药卫生—治疗学] R392-33[医药卫生—临床医学]