FGFR3在小鼠小肠发育阶段的作用  

The Effects of FGFR3 in Development Phase of Murine Small Intestine

作　　者：朱渝军[1] 黄显凯[2] 

机构地区：[1]解放军324医院普外科,重庆400020 [2]第三军医大学大坪医院创伤科

出　　处：《医学研究杂志》2010年第1期64-67,F0003,共5页Journal of Medical Research

基　　金：重庆市自然科学基金(CSTC-2006BB5056)

摘　　要：目的探讨FGFR3在小鼠小肠发育阶段的作用。方法观察同窝野生小鼠及FGFR3增强小鼠出生后小肠上皮形态学的变化及增生情况和FGFR3表达变化。结果FGFR3增强小鼠绒毛分布密度及绒毛高度均差于同年龄野生小鼠,但隐窝深度却是相反的。增生的细胞主要位于肠隐窝部位,FGFR3增强突变小鼠在各时相点细胞增生均强于野生小鼠。小鼠出生后1天小肠即有FGFR3表达,7～21天表达较多,35天后表达减少。免疫组化检测FGFR3表达位于肠隐窝部位,与Brdu表达信号部位相重叠。结论FGFR3在发育阶段促进小鼠肠隐窝形成。ObjectiveTo investigate the effect of fibroblast growth factor receptor 3（FGFR3） in development phase of murine small intestine.MethodsWild type mice and their littermate FGFR3＋ mice were used to observe their morphology and proliferation characteristics of epithelia of small intestinine in development phase and changes of FGFR3 expression.ResultsMutant mice had lower density and their villa were lower than those of the controls.But to the depth of crypt,the results were conversely.Cells in proliferation mainly located in intestine crypt.Mutant mice had more proliferation than the controls at every time point.Expression of FGFR3 was detected at birth（day 1）,and the expression was maximal from day7 to day 21,decreasing rapidly thereafter to reach the relatively low at day35.And the expression of FGFR3 also located in intestine crypt,overlapping with that of Brdu.ConclusionFGFR3 improves formation of murine intestine crypt in development phase.

关 键 词：FGFR3 增生小肠 

分 类 号：R681.3[医药卫生—骨科学] R734.2[医药卫生—外科学]