柴胡皂甙-d对人肝癌HepG2细胞增殖及细胞周期的影响  

Effects of saikosaponins-d on proliferation and cell cycle of HepG2 cell line

作　　者：张国平[1] 祝葆华[1] 王兰天[1] 童传明 敬敏[3] 

机构地区：[1]广东医学院附属医院普外科,广东湛江524001 [2]湖北省武汉市东西湖区人民医院外一科,湖北武汉430040 [3]广东医学院病理学教研室,广东湛江524023

出　　处：《广东医学院学报》2009年第6期603-605,共3页Journal of Guangdong Medical College

基　　金：广东省科技计划资助项目(No.2008B030301016)

摘　　要：目的探讨柴胡皂甙-d(SSd)对人肝癌HepG2细胞增殖和细胞周期的影响。方法(1)常规培养人肝癌HepG2细胞;(2)噻唑蓝(MTT)实验观察不同质量浓度SSd处理HepG2细胞72 h后以及10mg/L的SSd作用HepG2细胞不同时间对HepG2细胞生长指数的影响;(3)流式细胞术(FCM)检测SSd处理HepG2细胞48h后细胞周期的分布。结果SSd能明显抑制HepG2细胞的增殖,以10mg/L的SSd作用48h的抑制作用最明显。FCM检测显示SSd作用48h后实验组处于S期的细胞数较对照组明显减少(P<0.01)。结论SSd对人肝癌HepG2细胞的体外增殖具有抑制作用,并能使细胞周期中处于S期的细胞数减少。Objective To study the effects of saikosaponins-d（SSd） on proliferation and cell cycle in the human hepatocellular carcinoma cell line,HepG2 cell line.Methods HepG2 cells were cultured and treated with SSd.The cell growth rate was measured by monotetrazdium（MTT） assay and the cell cycle was determined by the flow cytometry（FCM） analysis.Results The SSd obviously inhibited the proliferation of HepG2 cell line in a concentration and time dependent manner,the maximum response of the cells to SSd was at the concentration of 10mg/L and the treatment time-point of 48h.Compared with the control,the cells treated with 10mg/L SSd for 48h significantly reduce cell numbers in S phase （P〈0.01）.Conclusion The SSd could inhibit the proliferation of HepG2 cell line and arrest its cell cycle in S phase.

关 键 词：柴胡皂甙-D HEPG2 增殖 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]