Survivi n-si RNA抑制肺癌A549细胞的增殖并增强其对顺铂的敏感性  

Survivin-siRNA inhibits proliferation of lung cancer A549 cells and enhances their chemosensitity to cisplatin

作　　者：张曙光[1] 刘晓凡[1] 杜江[1] 李文雅[1] 张林[1] 

机构地区：[1]中国医科大学附属第一医院胸外科,辽宁沈阳110001

出　　处：《中国肿瘤生物治疗杂志》2009年第6期583-587,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.30371624)~~

摘　　要：目的:构建靶向存活素(survivin)的干扰RNA(siRNA)质粒,观察其对A549细胞增殖、凋亡以及顺铂敏感性的影响。方法:应用pSilencer-U6质粒构建survivin-siRNA干扰质粒,RT-PCR和Western blotting检测survivin mRNA和蛋白的表达,DAPI染色法检测细胞的凋亡,MTT法检测细胞的增殖。结果:成功构建了survivin-siRNA干扰质粒。Survivin-siRNA质粒转染可明显下调A549细胞中survivin mRNA和蛋白的表达、抑制A549细胞的增殖、促进细胞的凋亡、增强A549细胞对顺铂的敏感性。结论:Survivin-siRNA沉默survivin在肺癌细胞中的表达能够抑制肿瘤细胞的增殖、促进细胞凋亡,并能增强肿瘤细胞对化疗药物顺铂的敏感性,survivin可作为肺癌治疗的潜在靶点。Objective：To investigate the effect of survivin-siRNA plasmid on survivin expression in human lung cancer cell line A549, and to observe its effect on the apoptosis, proliferation, and chemosensitivity of A549 cells. Methods： pSilencer-survivin-siRNA （survivin-siRNA） plasmid was constructed using pSilencer-U6 plasmid and was transfected into A549 cells. Expression of survivin mRNA and protein was examined by RT-PCR and Western blotting analysis, respectively. Apoptosis and proliferation of A549 cells were examined by DAPI staining and MTT, respectively. Results： Survivin-siRNA plasmid was successfully constructed, and it significantly inhibited survivin mRNA and protein expression in A549 cells. Survivin-siRNA transfection induced apoptosis, inhibited proliferation and increased chemosensitivity of A549 cells to cisplatin. Conclusion： pSilencer-survivin-siRNA can silence survivin expression in A549 cells and subsequently inhibit proliferation, promote apoptosis, and enhance chemosensitivity of A549 cells to cisplatin. Survivin may serve as a potential target for gene therapy of lung cancer.

关 键 词：存活素 RNA干扰 肺肿瘤 A549细胞 顺铂 

分 类 号：R734.2[医药卫生—肿瘤] R730.54[医药卫生—临床医学]