MTT比色法检测MGC803^T细胞毒效应的研究  

MTT Colorimetric Assay for Testing Cytotoxic Activity of MGC 803 T

作　　者：胡荫[1,2] 尹维刚[1,2] 许佐良 

机构地区：[1]包头医学院基础部 [2]北京医科大学

出　　处：《包头医学院学报》1998年第3期1-2,共2页Journal of Baotou Medical College

摘　　要：用插入突变的膜结合型ＴＮＦ—α基因的重组逆转录病毒载体转染人胃癌细胞ＭＧＣ，获得膜结合型ＴＮＦ—α基因转染阳性的人胃癌细胞ＭＧＣ８０３（ＭＧＣ８０３Ｔ）。用ＭＴＴ比色法、３Ｈ—ＴｄＲ释放法检测ＭＧＣ８０３Ｔ及未转染的人胃癌细胞ＭＧＣ８０３（ＭＧＣ８０３Ｎ）细胞表面ＴＮＦ—α的细胞毒效应。结果表明，ＭＧＣ８０３Ｔ对鼠纤维肉瘤细胞Ｌ９２９有杀伤作用，而对照细胞ＭＧＣ８０３Ｎ无杀伤作用，说明逆转录病毒介导的膜结合型ＴＮＦ—α基因在人胃癌细胞获得有效表达。二种方法检测ＭＧＣ８０３Ｔ细胞的细胞毒效应获得良好相关性。与３Ｈ—ＴｄＲ释放法相比，ＭＴＴ比色法具有操作简便。We used retroviral vector LXSN to construct recombinant retroviral vector with mutant membrane bound TNF-α gene.The retroviral vectors were used to transduce the human gastric cancer cell line,MGC 803 cells and positive colonies were obtained(named as MGC 803 T).MTT colorimetric assay and 3 H-TdR release assay were used to test the cellular cytotoxicity (MGC 803 T and control) related to TNF-α.The results showed that mutant TNF-α gene could be efficiently expression in the transduced human gastric cancer cells and MTT colorimetric assay may be used to test the cellular cytotoxicity.We think MTT colorimetric assay is a simple,rapid and safety method.

关 键 词：MTT比色法 细胞毒效应 肿瘤 MGC803 

分 类 号：R730.5[医药卫生—肿瘤]