抗HER-2工程抗体chA21对人卵巢癌SKOV3裸小鼠移植瘤血管生成的影响  

Effect of anti-HER-2 engineered antibody chA21 on angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells

作　　者：张安莉[1] 吴强[1] 杨枫[1] 沈国栋[2] 刘兢[2] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]中国科技大学生命科学院,合肥230027

出　　处：《临床与实验病理学杂志》2009年第6期627-630,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家"863"计划项目(2001AA215381)

摘　　要：目的探讨抗HER-2工程抗体chA21对过表达HER-2的人卵巢癌细胞株SKOV3裸小鼠移植瘤血管生成的影响。方法建立人卵巢癌SKOV3裸小鼠移植瘤模型,随机分成阴性对照组和chA21组,连续5周尾静脉注射给药,观察肿瘤生长变化;5周后处死裸鼠取下瘤体,利用组织芯片及免疫组化方法结合显微图像分析系统定量检测VEGF、DLL4表达情况,并用CD31免疫组化染色比较两组微血管密度(MVD)值。结果chA21组肿瘤组织中VEGF、DLL4的表达及MVD值均显著低于阴性对照组(P<0.05)。结论抗HER-2工程抗体chA21能显著抑制人卵巢癌SKOV3裸小鼠移植瘤的血管生成。Purpose To investigate the effect of anti-HER-2 engineered antibody chA21 on the angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells that overexpresses HER-2.Methods An animal model with SKOV3 cells involved in nude mice was established and the mice were randomized into control group and chA21 group;chA21 was administrated via caudal vein for 5 weeks,meanwhile the same volume of chA21 buffer was injected in control group.The mice were terminated after 5 weeks and the xenografts dislodged,VEGF and DLL4 expression in two groups were quantitatively analyzed by immunohistochemistry on tissue microarray sections combined with a micro-image analyzing system,and microvessel density（MVD） values were compared between the two groups by immunohistochemical staining of CD31.Results The expression of VEGF,DLL4 and MVD values in chA21 group were lower than those in control group（P〈0.05）.Conclusion chA21 inhibits the angiogenesis of transplantations of human ovarian cancer SKOV3 cells effectively.

关 键 词：卵巢肿瘤 HER-2 CHA21 SKOV3 VEGF DLLA 血管生成 

分 类 号：R737.31[医药卫生—肿瘤]