恒磁场对阿霉素杀伤人乳腺癌细胞的协同作用  

Synergistic inhibitory effects of static magnetic fields and adriamycin on breast cancer cell line MCF-7 in vitro

作　　者：程婕[1] 徐巧玲[2] 申广浩[1] 吴小明[1] 罗二平[1] 

机构地区：[1]第四军医大学生物医学工程系军队卫生装备与计量学教研室,陕西西安710033 [2]第四军医大学护理系,陕西西安710033

出　　处：《第四军医大学学报》2009年第24期3006-3008,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(50377044);陕西省自然科学基金(2006C211)

摘　　要：目的:观察恒磁场联合阿霉素对人乳腺癌细胞MCF-7增殖和凋亡的影响及其机制.方法:MCF-7细胞经不同浓度的阿霉素和/或恒磁场(10mT)处理24h后,以MTT法检测不同条件下细胞生长受抑制情况;流式细胞术检测各组细胞周期分布;荧光显微镜检测细胞内活性氧变化情况;蛋白电泳检测凋亡相关蛋白Caspase3表达情况.结果:恒磁场可明显增强阿霉素对MCF-7细胞生长的抑制效应(P<0.01),更强地抑制细胞DNA合成及有丝分裂(P<0.05),促使细胞内活性氧合成,并上调凋亡相关蛋白Caspase-3的表达.结论:恒磁场(10mT)对阿霉素杀伤人乳腺癌细胞具有较强的协同作用.AIM：To explore the synergistic effects of static magnetic fields（SMF） and adriamycin（ADM） on cell growth in human breast cancer cell MCF-7 in vitro.METHODS：The MCF-7 cells were exposed to SMF in combination with various concentrations of ADM for 24 h.To analyze SMF effects in cell growth,MTT assay,flow cytometry,fluorescence microscopy and Western Blot analysis were used.RESULTS：SMF exposure significantly enhanced the cell growth inhibition and apoptosis by ADM,in which cell cycle arrest,cleaved Caspase-3,and reactive oxygen species（ROS） increased.CONCLUSION：SMF（10 mT） could enhance the antitumor effects of ADM,and would be a possibility of comprehensive therapy for breast cancer.

关 键 词：恒磁场 乳腺肿瘤 阿霉素 

分 类 号：R730.5[医药卫生—肿瘤]