Notch信号在急性早幼粒细胞白血病患者骨髓中的表达  

Notch signal expression in the bone marrow of patients with acute promyelocytic leukemia

作　　者：张丽[1] 夏德雨[1] 李国辉[1] 蒋姗姗[1] 谷芳娜[1] 梁英民[1] 

机构地区：[1]第四军医大学唐都医院血液科,陕西西安710038

出　　处：《第四军医大学学报》2009年第24期3052-3054,共3页Journal of the Fourth Military Medical University

基　　金："十一五"国家"863"重大;重点项目(2006AA02A111)

摘　　要：目的:通过检测急性早幼粒细胞性白血病(APL)患者治疗前后骨髓中Notch信号途径中受体、配体和靶基因的表达情况,探讨其在APL发生中的作用.方法:采用半定量RT-PCR技术检测APL患者治疗前后骨髓中Notch1,Notch2,Notch3,Notch4,Delta-like-l,Delta-like-4,Jagged1,Jag-ged2,Hes1,Hes5的表达.结果:受体Notch2、配体Jagged1、靶基因Hes1的表达在APL完全缓解后明显下调,其它受体、配体及靶基因的变化无统计学意义.结论:在APL患者治疗前后存在Notch基因的改变,Notch信号的这些改变在APL的发病机制中可能有重要作用.AIM：To explore the possible role of Notch signaling in occurrence of acute promyelocytic leukemia（APL） through investigate the expression of Notch receptors,ligands and target gene in the bone marrow of patients with APL.METHODS：Semi-quantitative reverse transcriptase-polymerase chain reaction（RT-PCR） was used to investigate the expression of Notch1,Notch2,Notch3,Notch4,Delta-like-l,Delta-like-4,Jagged1,Hes1,Hes5 in the bone marrow of patients with APL,and the changes of expression after treatment.RESULTS：The expressions of Notch2,Jagged1 and Hes1 were significantly lower after treatment,the expressions of the others have no significant difference.CONCLUSION：The changes of Notch2,Jagged1 and Hes1 expression after treatment are significant.The difference on the expression of Notch receptors and ligands may be important significance for the pathogenesis of APL.

关 键 词：NOTCH 急性早幼粒细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤]