白细胞介素-27基因多态性与结核病易感性的关系  

Association between interleukin 27 gene polymorphisms and susceptibility to tuberculosis

作　　者：战玉助[1] 蒋利萍[1] 王莉[1] 解元元[2] 朱朝敏[2] 郭述良[3] 

机构地区：[1]重庆医科大学附属儿童医院临床免疫研究室,重庆400014 [2]重庆医科大学附属儿童医院感染消化科,重庆400014 [3]重庆医科大学附属第一医院呼吸内科,重庆400016

出　　处：《第四军医大学学报》2009年第24期3100-3103,共4页Journal of the Fourth Military Medical University

摘　　要：目的:探讨重庆地区汉族人群白细胞介素-27(IL-27)基因多态性与结核病易感性的关系.方法:采用聚合酶链反应-限制性片段长度多态(PCR-RFLP)和序列特异性引物多聚酶链反应(PCR-SSP)分析385例结核病患者和391例健康对照的IL-27基因的-964A/G和2905T/G位点多态性,统计分析基因多态性与结核病易感性的关系.结果:IL-27基因-964位点存在A、G二态性,表现为AA纯合、GG纯合、AG杂合三种基因型,2905位点存在T、G二态性,表现为TT纯合、GG纯合、TG杂合三种基因型.IL-27基因的-964A/G位等位基因和基因型分布频率分别在结核组与对照组间的差异均有统计学意义(P<0.05),结核组的等位基因A频率(66.4%)明显高于对照组(59.1%)(χ2=8.80,P=0.003),结核组AA基因型频率(48.1%)明显高于对照组(38.1%)(χ2=7.83,P=0.005).-964A/G位等位基因和基因型分布频率在结核菌阳性患者组与结核菌阴性患者组间的差异亦有统计学意义(P<0.05),结核菌阳性组的A等位基因频率(70.8%)明显高于结核菌阴性组(61.1%)(χ2=8.11,P=0.004).2905T/G位基因型和等位基因分布频率在结核组与对照组间差异无统计学意义.结论:IL-27基因-964A/G多态性与结核病易感性相关,携带-964A等位基因的人群更易患结核病,结核分枝杆菌阳性率高,而2905位T/G多态性与结核病易感性无明显相关性.AIM： To investigate the correlation between the polymorphisms of interleukin-27 （ IL-27 ） gene and susceptibility to tuberculosis in the Chinese Han Population. METHODS： The polymorphisms of interleukin 27 （-964A/G, 2905T/G） in 385 patients with tuberculosis and 391 healthy control subjects was detected by using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and sequence specific primers polymerase chain reaction（PCR-SSP） , and their susceptibility to tuberculosis were analyzed. RESULTS： The A/G polymorphisms （ AA, AG and GG genotypes） at position -964 in promoter of IL- 27 gene and the T/G polymorphisms （ TT, TG and GG genotypes） at position 2905 of IL-27 gene were identified. Statistically significant differences in allele and genotype frequencies of the IL-27 （-964A/G） were found between patients and controls. The allele frequency of A of IL-27（-964A/G） in the patients （66.4%） was significantly higher than that of controls （ 59. 1% ） （ χ2 = 8.80, P =0. 003）. The genotypic frequency of AA of IL-27（-964A/G） in the patients（48.1% ） was significantly higher than that of con- trols （38.1%） （ χ2 = 7.83, P = 0. 005 ）. Statistically significant differences in genotype frequencies of the -964A/G were found between patients with positive yield of tuberculosis and patients with negative yield of tuberculosis. The allele frequency of A of - 964A/G in those with positive yield of tuberculosis（ 70.8% ） was significantly higher than those with negative yield of tuberculosis （61.1%） （χ2 =8.11, P=0.004）. There were no differences in allele and genotype frequencies of IL-27 （2905T/G） between patients and controls. CONCLUSION： This study suggested the association between IL-27 gene -964A/G polymorphisms and sus- ceptibility to tuberculosis, and with A allele is more susceptibility to tuberculosis and positive yield of tuberculosis. There is no association between IL-27 （2905T/G） polymorphisms and suscep- tibil

关 键 词：IL-27 基因多态性 结核病 易感性 

分 类 号：R52[医药卫生—内科学]