黄孢原毛平革菌锰过氧化物酶基因(MnP2)的克隆  

Molecular Clone of cDNA Encoding Manganese Peroxidase (MnP2) from Phanerochaete chrysosporium

作　　者：杨晓宽[1] 

机构地区：[1]河北科技师范学院食品科技学院,河北秦皇岛066600

出　　处：《河北科技师范学院学报》2009年第4期45-49,共5页Journal of Hebei Normal University of Science & Technology

摘　　要：应用RT-PCR技术,从黄孢原毛平革菌5.766(Phanerochaete chrysosporium)总RNA中成功扩增出预期大小约为1.3 kb的特异性条带,将扩增产物提纯后克隆入PUC19载体,经转化、筛选及酶切鉴定后,获得锰过氧化物酶(MnP2)基因的克隆。序列分析表明,扩增的MnP2基因片段其cDNA长度为1 149 bp,编码358个氨基酸,与其它已发表文献报道的MnP2序列一致,与其同工酶MnP1和MnP3的核苷酸同一性分别为81%和66%。cDNA encoding specialized fragment about 1. 3 kb was amplified successfully by RT-PCR from the total RNA of Phanerochaete chrysosporium 5. 776 and cloned into puc19 vector for sequence after purification. The MnP2 gene cloning was obtained after transformation, screening and enzyme-cut identification. The sequence analysis showed that the cDNA nucleotide sequence of the MnP2 was 1 149 bp and encoded a protein of 358 amino acids. Sequence comparison with other published MnP2 genes showed that the nucleotide identity was 100% while sequence comparison with MnP1 and MnP3 gene Showed that the nucleotide identity was respectively 81% and 66%.

关 键 词：锰过氧化物酶 CDNA 同工酶序列 反转录聚合酶链式反应 

分 类 号：Q936[生物学—微生物学]