OPN反义基因对乳腺癌MDA-MB-231细胞迁移能力的影响  

Effect of anti-sense gene of osteopontin on migration ability of MDA-MB-231 cells of breast cancer

作　　者：姜伟栋[1] 印芳颖[2] 宫路路[1] 张厚忠[1] 乔士兴[1] 

机构地区：[1]吉林大学第二医院基本外科,吉林长春130021 [2]吉林大学中日联谊医院儿科

出　　处：《中国妇幼保健》2010年第4期537-539,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨OPN反义基因(ANOPN)对人乳腺癌细胞系MDA-MB-231细胞生长及迁移能力的影响。方法:构建人骨桥蛋白反义基因真核表达质粒pcDNA3.1-ANOPN,将质粒pcDNA3.1-ANOPN、空载体pcDNA3.1(+)和等量的脂质体分别转染人乳腺癌细胞系MDA-MB-231,将转染细胞分别命名为MDA-ANOPN、MDA-vect和MDA,在体外观察各组细胞的迁移能力。结果:MDA-MB-231细胞比MDA-vect及MDA细胞迁移能力弱(P<0.01)。结论:ANOPN可抑制人乳腺癌细胞系MDA-MB-231细胞的迁移能力。Objective： To explore the effect of anti - sense gene of osteopontin （ANOPN） on migration ability of MDA - MB - 231 cells of breast cancer. Methods： Eukaryotic expression vector pcDNA3. 1 - ANOPN was constructed, then pcDNA3.1 - ANOPN, empty vector pcDNA3.1 （ ＋ ） and commensurable liposome were used to transfect human breast cancer cell line MDA - MB - 321 cells, and the transfected cells were named as MDA - ANOPN, MDA - vect and MDA, respectively. The migration ability of these cells were observed. Results ： The migration ability of MDA - MB - 231 cells was stronger than those of MDA - vest cells and MDA cells （ P 〈 0. 01 ） . Conclusion： ANOPN can suppress the migration ability of human breast cancer cell line MDA -MB -231 cells.

关 键 词：骨桥蛋白 反义基因 乳腺癌 迁移能力 

分 类 号：R737.9[医药卫生—肿瘤]