红细胞生成素3'-增强子调节缺氧时内皮细胞血栓素合酶基因表达  

ERYTHROPOIETIN 3′—ENHANCER REGULATES EXPRESSION OF THROMBOXANE SYNTHASE IN HYPOXIC ENDOTHELIAL CELLS

作　　者：郝天玲[1] 夏若寒[1] 于江洲[1] 

机构地区：[1]同济医科大学病理生理学教研室 [2]卫生部呼吸系疾病重点实验室

出　　处：《高原医学杂志》1998年第3期5-8,共4页Journal of High Altitude Medicine

基　　金：国家自然科学基金

摘　　要：目的：研究缺氧对人脐静脉内皮细胞珠ＥＣ－３０４ＴＸＳ基因的影响及ＥＰＯ３′增强子在其中的作用。方法：将红细胞生成素（ＥＰＯ）３′－增强子野生片断及点突变片断，借脂质体转入人脐静脉内皮细胞株ＥＣ－３０４，用半定量ＲＴ－ＰＣＲ测定常氧与缺氧条件下培养６小时的细胞血栓素合酶（ＴＸＳ）ｍＲＮＡ。结果：ＥＣ－３０４常氧培养有ＴＸＳ基因表达缺氧６小时ＴＸＳ基因表达明显增强；而细胞导入野生ＥＰＯ３′－增强子片断可阻断缺氧诱导ＴＸＳ基因表达增强，而点突变片断无此作用。结论：在ＴＸＳ基因序列中，可能存在类似ＥＰＯ３′－增强子片断，缺氧诱导ＴＸＳ基因表达增强可能主要通过缺氧诱导因子－１与ＴＸＳ基因序列中类似ＥＰＯ３′－增强子片断结合。Objective:To investigate whether erythropoietin 3′-enhancer regueates expression of thromoxane synthase in hypoxic endothelial cells.Mechods:Cultured human umbilical vein endothelial cell line EC-304 was tranfected by wild or spot mutant fragments of erythropoietin(EPO) 3′-enhaner DNA with the help of liposome.Total RNA was extracted from EC-304 exposed to normoxia or hypoxia for 6 hours,The amount of TXS mRNA was measured by semiquantitation RT-PCR.Results:There was an expression of TXS gene in normoxic EC-304 cells,it was increasedin EC-304 cells exposed to hypoxia for 6 hours,which could be inhibited by tranfection of the wild EPO 3′-enhancer fragment but not by the mutant one. Conclasions:There might be a EPO 3′-enhancer-like sequence in TXS gene,which in combination with hypoxia-induced factor-1 might cotribute to the increase of TXS mRNA induced by hypoxia.

关 键 词：缺氧 血栓素合酶 基因表达 EC-304TXS 

分 类 号：R364.4[医药卫生—病理学]