甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8,9蛋白活性变化及凋亡机制研究  

Changes in caspase-3,8,9 activities of normal human hepatocyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate

作　　者：传良敏[1] 杨永长[1] 黄文芳[1] 饶绍琴[1] 洪华[1] 邓君[1] 

机构地区：[1]四川省人民医院检验科,四川成都610072

出　　处：《四川医学》2010年第1期10-12,共3页Sichuan Medical Journal

摘　　要：目的观察甘氨脱氧胆酸盐(glycodeoxycholate,GCDC)诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8,9活性的变化,探讨其凋亡机制。方法体外培养HL-7702细胞,以GCDC为处理因素,用AnnexinV-FITC/PI双染色法结合流式细胞技术仪检测细胞凋亡率,比色法测定caspase-3,8,9活性。结果100、150、200、250μmol/L的GCDC处理HL-7702细胞24h后以及250μmol/LGCDC处理细胞4、8、12、24h后,其细胞凋亡率、caspase-3,8,9活性明显升高,与对照组比较,差异有统计学意义(P<0.05)。结论GCDC可通过活化caspase-8,9,进而激活caspase-3从而诱导肝细胞凋亡。Objective To investigate the apoptosis and changes of caspase-3,8,9 activities in cultured normal human hepatocyte HL-7702 cells induced by glycodeoxycholate（GCDC） in vitro and to explore the possible mechanism of hepatocyte apoptosis. Methods HL-7702 cells were cultured with GCDC. The apoptosis ratio of HL-7702 cells was determined by Annexin VFITC/PI double staining. Colorimetric method was used to measure caspase-3,8,9 activities. Results When hepatocytes were incubated with GCDC for 24h,the apoptotic ratio ,caspase-3,8,9 activities of HL-7702 cells increased remarkably and had concentration-dependent and time-dependent with GCDC. Conclusion HL-7702 cells could be induced to apotosis by GCDC. The un- derlying mechanism might be related to up-regulation of caspase-8,9 activities which subsequently activated caspase-3 to induce HL-7702 cells apoptosis.

关 键 词：甘氨脱氧胆酸盐 细胞凋亡 CASPASE 

分 类 号：R341[医药卫生—基础医学]