内毒素诱发小鼠肝细胞凋亡的观察  

The dynamic changes in hepatic cell apoptosis induced by endotoxin in mouse

作　　者：徐新女[1,2] 周云丽 吴胜群[1,2] 李瑾[1,2] 王学谦 

机构地区：[1]天津市第一中心医院 [2]天津市肿瘤医院

出　　处：《中国危重病急救医学》1998年第10期618-620,共3页Chinese Critical Care Medicine

摘　　要：目的：观察内毒素攻击诱导小鼠肝细胞凋亡及与肿瘤坏死因子α（ＴＮＦα）的关系。方法：用精制内毒素腹腔注射于实验小鼠，分别于不同时间点测其血清中ＴＮＦα、谷丙转氨酶（ＧＰＴ）和乳酸脱氢酶（ＬＤＨ）含量，同时取肝脏制成组织切片进行ＨＥ和末端脱氧核糖转移酶介导的生物素化脱氧尿嘧啶缺刻标记技术（ＴＵＮＥＬ）染色，分别于光镜及荧光显微镜下观察肝细胞凋亡情况。结果：在内毒素攻击下，小鼠血清ＴＮＦα于９０分钟时首先达到峰值（１２９１．５０±１３４．４０）ｎｇ／Ｌ，其后即下降；２小时时肝细胞出现明显凋亡现象，肝组织坏死及血清酶学改变于４小时才明显产生。结论：内毒素首先诱导ＴＮＦα等释放。Objective:To observe the dynamic changes in hepatic cell apoptosis induced by endotoxin and their correlation to tumor necrosis factorα(TNFα) release.Methods:Endotoxin was peritoneally injected to induce hepatic cells apoptosis in mouse.Serum TNFα,glutamic pyruvic transaminase,and lactate dehydrogenase levels were measured at various intervals.Meanwhile,liver tissue section was performed with HE and terminal deoxynucleotidy transferase mediated dUTPbiotin nickend labeling(TUNEL) staining,and hepatic cell apoptosis was identified under light microscope and fluorescence microscope.Results:Following endotoxin challenge,serum TNFα in mouse reached a peak value at 90 minutes (1 291 50±134 40)ng/L ,then it declined sharply.A significant increase in hepatic cell apoptosis was found at 2 hours after endotoxin injection,while hepatic necrosis together with remarkable elevation in serum enzymes occurred 4 hours later.Conclusions:These data suggest that endotoxin first induces the release of TNFα,and TNFα together with other cytokines initiate the process of apoptosis in hepatic cells.

关 键 词：内毒素 细胞凋亡 肝脏 小鼠 

分 类 号：R363.14[医药卫生—病理学]