ABA和GA刺激的ROS代谢调节水稻幼根伸长分析  

Analysis of ABA-and GA-stimulated Reactive Oxygen Species Mediating the Elongation of Rice Seeding Roots

作　　者：张幸福[1] 韩栓[1] 王伟[1] 江静[1] 

机构地区：[1]河南大学植物逆境生物学重点实验室,河南开封475004

出　　处：《河南大学学报（自然科学版）》2010年第1期62-66,共5页Journal of Henan University:Natural Science

基　　金：河南省杰出青年支持计划项目资金资助(084100510022)

摘　　要：本文探索水稻幼根生长过程中ABA和GA对ROS代谢的影响,结果显示:低于0.5μmol/L的ABA促进根伸长,超过此浓度的ABA抑制根伸长;H2O2与ABA相似,低浓度促进而高浓度抑制;在观察浓度范围内,GA促进根伸长.利用3,3'-二氨基联苯胺(diaminobenzidine,DAB)组织化学染色方法检测根尖部位H2O2代谢情况,结果显示,对照、ABA,GA和ABA+GA处理后,根尖分别呈现浅黄色、深棕色、黄色和深棕色,说明ABA比GA诱导较多H2O2产生.进一步研究发现,ABA和ABA+GA分别上调过氧化物酶(peroxidase,POD)活性13.4%和8.37%,GA下调POD活性19.0%;同时,ABA和ABA+GA处理分别下调过氧化氢酶(catalase,CAT)活性14.8%和16.3%,GA则上调CAT活性5.16%.以上结果表明,ABA可能通过上调POD和抑制CAT活性来提高ROS含量,而GA与之相反,严谨控制ROS浓度,促进水稻生长.The results show that less than 0.5 umol/L ABA accelerates the rice seeding root elongation, but more than 0.5 umol/L ABA turns to inhibit the process. Similar to ABA, H2O2 accelerating turns to inhibiting the rice seeding root elongation at 1mmol/L concentration. GA accelerating rice root elongation in the all concentration is used. By diaminobenzidine （DAB） stains to detect the H2O2 accumulation in rice seeding roots, the results show that H2O2 accumulation are intensified by ABA or ABA ＋GA treatment but not by GA. Moreover, ABA and ABA ＋GA up-regulated the activity of peroxidase （POD） in 13.4% and 8.37%, respectively, but GA down-regulated the activity of POD in 19.0%. Moreover, the activity of catalase （CAT） is strongly down-regulated by ABA and ABA ＋ GA in 14.8% and 16.3%, respectively, but GA up-regulated the activity of CAT in 5.16%. In a word, ABA and GA control the activities of both POD and CAT and thus regulating the H2 O2 accumulation in rice seeding roots, suggesting that ABA and GA may modulate plant growth and development by mediating the ROS metabolism.

关 键 词：GA ABA ROS POD CAT 

分 类 号：Q505[生物学—生物化学]