细菌DNA图谱分析幽门螺杆菌阳性病人治疗后的复发和再感染  

Analyses of recrudescence and reinfection of Helicobacter pylori infection after antimicrobial treatment by DNA fingerprinting

作　　者：华杰松[1,2,3,4] 夏华向[1,2,3,4] 郑鹏远[1,2,3,4] 

机构地区：[1]新加坡国立大学医学院微生物学系 [2]悉尼大学医学系 [3]法国Bordeaux第二大学细菌学系 [4]河南医科大学二附院消化内科

出　　处：《胃肠病学和肝病学杂志》1998年第3期209-211,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的：分析和比较幽门螺杆菌（Ｈｐ）感染病人前和治疗后胃内分离到的细菌ＤＮＡ图谱。方法：用随机扩增多形态ＤＮＡ技术（ＲａｎｄｏｍａｍｐｌｉｆｉｅｄｐｏｌｙｍｏｒｐｈｉｃＤＮＡ，ＲＡＰＤ）检测９对Ｈｐ菌株，多聚酶链反应采用１０个碱基对的随机引物。结果：不同Ｈｐ菌株ＤＮＡ图谱显示极大差异性。６对细菌ＤＮＡ图谱治疗前与治疗后相同，提示病人感染同一细菌，１对细菌ＤＮＡ图谱治疗前与治疗后相似，提示病人体内Ｈｐ的ＤＮＡ经抗生素治疗后可能发生变异，２对细菌ＤＮＡ图谱治疗前与治疗后显著不同，提示病人感染不同细菌。结论：Ｈｐ感染病人经抗生素治疗转阴后若又重复感染，多数情况是病人感染同一Ｈｐ菌株，有少数病人再感染不同株Ｈｐ。Aim: To analyse DNA fingerprintings of H.Pylori isolates by polymerase chain reaction based random amplified polymorphic DNA (RAPD) and compare DNA profiles of paired strains isolated before and after treatment. Materials and methods: Nine pairs of H.pylori strains were isolates from patients with gastroduodenal disease. The second or third strains of each pair were isolated between 5 and 50 months after the first strain. RAPD fingerprinting was performed using a random primer with 10 base pairs. DNA profiles of H.pylori were analysed.Results: The RAPD fingerprinting demonstrated the highly heterogeneity of H.pylori.Among the 9 pairs of strains,6 gave the same DNA profiles. Two pairs of H.pylori strains collected at an interval of 18 and 23 months showed different DNA profiles. One pair of H.pylori strains gave similar DNA profile. Conclusion:Recrudescence of H.pylori infection after antimicrobial treatment was the main reason of relapse of H.pylori infection.Reinfection of H.pylori was also observed.

关 键 词：幽门螺杆菌 NDA图谱 胃炎 治疗 再感染 

分 类 号：R573.3[医药卫生—消化系统]