HPLC法测定阔叶五层龙中儿茶素类成分的含量  

Determination of Catechin Compound in Salacia amplifolia by HPLC

作　　者：濮江[1] 冯锋[1] 谢宁[1] 

机构地区：[1]中国药科大学天然药物化学教研室,江苏南京210009

出　　处：《中国中医药信息杂志》2010年第1期47-48,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的采用HPLC法测定阔叶五层龙中(-)-表儿茶素-(4β→8)-(-)-4'-O-表没食子儿茶素(ECEGC)的含量。方法用HYPERSILC18柱,以甲醇-水(30∶70)为流动相,用紫外检测器于283nm波长处检测。结果ECEGC线性范围为0.4448～7.1168μg(r=0.9999),平均回收率为100.30%。结论本法具有操作简便,分析快速准确、干扰小等优点。Objective To determine the content of ECEGC in Salacia arnplifolia by HPLC. Method HYPERSIL C18 column was used with a mixture of methanol and water （30 ： 70） as mobile phase, and detected at 283 nm. Result The linear range was 0.444 8-7.116 8 μg （r =0.999 9） for ECEGC. The average recovery was 100.30%. Conclusion The method is simple, rapid, accurate and reliable.

关 键 词：阔叶五层龙 儿茶素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]