重症肌无力患者神经肌肉接头处的超微结构研究  

Ultrastructural study on the neuromuscular junction of patients with myasthenia gravis

作　　者：宋东林[1,2] 石进[1,2] 刘翠兰[1,2] 张宏 吕强[1,2] 陈晋文 李恭[1,2] 

机构地区：[1]北京空军总医院神经内科 [2]吉林医学院附属医院神经内科

出　　处：《中华神经科杂志》1998年第5期299-301,共3页Chinese Journal of Neurology

摘　　要：目的探讨重症肌无力（ＭＧ）时神经肌肉接头（ＮＭＪ）突触后、前膜的变化及其意义。方法肋间内肌在辣根过氧化酶标记的α银环蛇毒素（ＨＲＰαＢｕＴｘ）标记后，用图形扫描及医学图像分析软件测量电镜照片中ＮＭＪ的各项指标，并与对照组比较分析。结果ＭＧ时突触后膜长度缩短，后、前膜长度比值变小，神经末端面积及其与突触后膜面积之比变小，突触后膜上的乙酰胆碱受体（ＡＣｈＲ）减少了３５．９％。结论ＭＧ时ＮＭＪ的变化主要存在于突触后膜。突触后、前膜长度之比对本病的诊断更有临床意义。Objective To further study the pathological characteristics at pre and post synaptic membranes of the neuromuscular junction (NMJ) in patients with myasthenia gravis(MG) and their significance. Method The NMJ of biopsied internal intercostal muscles was labeled by HRP α BuTx and photographed under the electron microscope. Some morphological parameters were quantitatively and statistically analyzed with image and software analytic system and compared with normal controls. Results The length of postsynaptic membrane decreased obviously. The ratio of the length of postsynaptic with presynaptic membrane, and that the motor nerve terminal area with postsynaptic membrane area diminished. The AChR on the postsynaptic membrane decreased by 35.9 percent as well. Conclusion The pathological changes at NMJ in MG patients mainly occurred in postsynaptic region. It is helpful to diagnose such a disease by investigating the ratio of the length of post/presynaptic membrane.

关 键 词：重症肌无力 神经肌肉接头 突触 免疫组织化学 

分 类 号：R746.1[医药卫生—神经病学与精神病学]