“房室不节”所致肾虚证动物模型造模方法探讨  

To Explore Method of Modeling Kidney Deficiency Syndrome Animal Model Due to Intemperate Sexual Intercourse

作　　者：谢玲玲[1] 王萧[1] 张永斌[1] 

机构地区：[1]广州中医药大学

出　　处：《山西中医》2010年第2期46-48,共3页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的:探讨单纯房室不节动物模型的造模方法。方法:以李震创立的"劳倦过度,房室不节"肾虚模型作为对照,分别设立4周、8周、12周3个"房室不节"造模组进行造模,比较各组的精子活动率和计数,精子畸形率,母鼠怀孕胚胎数;睾丸的脏器称重及病理改变、睾丸乳酸脱氢酶X同功酶(LDH-X)相对活性。结果:"劳倦过度,房室不节"组精子活动率和密度、精子畸形率、母鼠怀孕胚胎数、睾丸重量及系数、LDH-X相对活性与空白对照组比较,有统计学意义;"房室不节"4周组、8周组试验参数接近空白对照组,12周组接近"劳倦过度,房室不节"组。结论:单独使用"房室不节"方法进行12周造模,能有效建立"房室不节"致肾虚证"肾主生殖"功能虚损雄性小鼠模型。Objective ：To explore how to make the animal model of intemperate sexual intercourse. Method： Kidney deficiency model caused by＇ overstrain and intemperate sexual intercourse＇ and created by LI Zhen was as a control, three groups of intemperate sexual intercourse were set up for modeling, which were 4 weeks group, 8 weeks group and 12 weeks group. Such data were compared as the sperm motility and count, sperm deformity rate, the number of female mouse embryos pregnancy, testes weighing and pathological changes, and relative activity of testicular lactate dehydrogenase X isozyme （LDH-X）. Result：There was statistical significance between the group of intemperate sexual intercourse and blank control group on sperm motility and count, sperm deformity rate, preg- nant female mouse embryos numbers, the testes weight and coefficient, LDH-X relative activity. Test parameters of 4-week group and 8-week group were close to those of blank control group, 12-week group close to the group of in- temperate sexual intercourse. Conclusion：Modeling the model of intemperate sexual intercourse for 12 weeks can effectively establish male mouse models of functional defficiency caused by intemperate sexual intercourse leading to kidney deficiency syndrome, because of ＇kidney controlling reproduction＇ .

关 键 词：房室不节 小鼠 肾虚 实验研究 

分 类 号：R228[医药卫生—中医基础理论]