丙酮酸乙酯对LPS致急性肺损伤大鼠肺组织NF-κB p65及TNF-α和IL-lβ的影响  

Effects of Ethyl Pyruvate on the Expression of NF-κB p65 and TNF-α,IL-lβ in Rat with Lung Injury Induced by Endotoxin

作　　者：郑翔[1] 季艳梅[1] 郭学珍[1] 黄云飞[1] 陈黎[1] 

机构地区：[1]郧阳医学院附属十堰市太和医院中心ICU科,442000

出　　处：《微循环学杂志》2010年第1期4-6,共3页Chinese Journal of Microcirculation

基　　金：湖北省科技攻关项目(NO:2004AA301C97)

摘　　要：目的:观察丙酮酸乙酯(EP)对内毒素性急性肺损伤(ALI)大鼠肺组织核转录因子-κB(NF-κB)p65表达及肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量的影响,探讨EP可能的肺保护机制。方法:雄性SD大鼠30只随机分为三组(n均=10):正常对照组,静脉注射与其它二组等量生理盐水;LPS组,静脉注射脂多糖(LPS)5mg/kg复制大鼠ALI模型;EP+LPS组,于静脉注射LPS前1h腹腔内注射EP(40mg/kg)。所有动物于注射LPS或生理盐水后4h颈动脉放血处死,取肺组织用Westernblot测定其NF-κBp65的表达,用ELISA测定其TNF-α和IL-1β的含量。结果:与对照组相比,LPS组、EP+LPS组肺组织NF-κBp65表达增加,肺组织TNF-α和IL-lβ含量升高(P<0.05);与LPS组相比,EP+LPS组肺组织NF-κBp65表达降低,肺组织TNF-α和IL-lβ含量降低(P<0.05)。结论:EP通过下调大鼠LPS诱导的肺组织NF-κBp65表达,降低了TNF-α和IL-lβ的释放。EP可减轻ALI大鼠肺的炎症反应。Objective:To investigate the effects of ethyl pyruvate(EP)on the expression of nuclear factor-kappaB(NF-κB)p65 in the lungs induced by lipopolysaccharide(LPS).Method:The rat model of acute lung injury was established via femoral vein injection LPS(5mg/kg).Thirty male SD rats were randomly divided into 3 groups(n=10 each):control group;LPS group;EP+LPS group.Control group received physiologic saline 30ml/kg only,while LPS group received 0.1% LPS 5mg/kg and physiologic saline 30ml/kg by right femoral vein injection.The treatment of EP+LPS group was same with LPS group except that it pretreated with intrapentoneal EP(40mg/kg)before 1 h of LPS injection.The animals were bled to death at 4h after LPS administration.The lungs were immediately removed for determination of expression of NF-κB p65(Western blot)and TNF-α and IL-lβ content(ELISA).Results:Compared with control group,the expression of NF-κB p65 in the cell nucleus and the content of TNF-α and IL-lβ in the lungs was significantly increased in LPS group and EP+LPS group;while compared with LPS group,they were significantly decreased in EP+LPS group(P<0.05).Conclusion:EP can attenuate the changes in NF-κB p65 expression and TNF-α and IL-lβ content in the lung induced by LPS.EP may alleviate the inflammation of acute lung injury in rat.

关 键 词：NF-ΚB 急性肺损伤 大鼠肺组织 丙酮酸乙酯 TNF-α LPS P65 急性呼吸窘迫综合征 

分 类 号：R363[医药卫生—病理学] R563[医药卫生—基础医学]