5-氨基乙酰丙酸介导的光动力学治疗人脑胶质瘤  

Photodynamic therapy mediated by 5-aminolevulinic acid for U251 human glioma

作　　者：许焕波[1] 肖哲[1] 袁军[1] 

机构地区：[1]汕头大学医学院第一附属医院神经外科,广东汕头515041

出　　处：《广东医学》2010年第2期146-149,共4页Guangdong Medical Journal

基　　金：广东省医学科学技术研究基金资助课题(编号:WSTJJ20051210)

摘　　要：目的探讨光敏剂5-氨基乙酰丙酸介导光动力学疗法(photodynamic therapy,PDT)对体外培养的U251细胞株的疗效。方法收集对数期生长的U251细胞,用台盼蓝染色细胞计数法确认细胞存活率在95%以上,接种于96孔板上,每孔含细胞数:5 000～10 000个,并分别以不同累积能量[每孔累积能量分别为(15、30、45、60、75)J/cm2]、不同浓度的5-氨基乙酰丙酸(浓度分别为0、0.5、1.0、1.5、2.0、2.5、3.0 mmol/L)介导PDT,光辐照后各孔补加50μL含10%新生牛血清的培养液,继续培养24 h。使用CCK-8法测定各孔细胞数并计算细胞存活率。结果(1)5-氨基乙酰丙酸本身对U251细胞无细胞毒性作用。(2)单孔累积能量45 J/cm2、5-氨基乙酰丙酸浓度为2.5 mmol/L时,PDT效果最好,U251细胞生存率约为50.2%。结论5-氨基乙酰丙酸介导的PDT对U251细胞有杀伤作用。Objective To study the efficacy of photodynamic therapy （PDT） mediated by photosensitizer 5 - aminolevulinic acid （5 - ALA） for U251 cell line in vitro. Methods U251 cells at logarithmic growth were planted in 96 - well plates, with 5000 - 10000 cells per well. Cell viability was confirmed by trypan blue stain with more than 95% viable cells. Groups of U251 cells were irritated with various accumulated energy （0,15,30,45,60,75 J/cm^2） mediated by different concentrations of 5 -aminolevulinic acid （ 0 mmol/L, 0. 5 mmol/L, 1.0 mmol/L, 1.5 retool/L, 2.0 mmol/ L, 2. 5 mmol/L, 3.0 mmol/L） ,and incubated in 10% newborn calf serum culture medium for 24 hours. Cell Counting Kit -8 （CCK -8 ） assay was use to assess the survival rate in wells. Results 1. There was no cell toxicity effect on U251 cells of 5 - ALA alone. 2. The PDT with accumulated energy of 45J/cm^2 and mediated by 5 - aminolevulinic acid concentration of 2. 5 mmol/L achieved an optimal therapeutic effect with a minimal survival rate of 50.2%. Conclusion PDT mediates by 5 - ALA effected kills U251 cells in vitro.

关 键 词：光动力学疗法 光敏剂 5-氨基乙酰丙酸 U251 

分 类 号：R735.35[医药卫生—肿瘤] R454.2[医药卫生—临床医学]