青光眼动物模型DBA/2J小鼠的眼部特征及组织学观察  

Ocular findings and histological change in glaucoma model of DBA/2J mice

作　　者：杨帆[1] 吴玲玲[1] 郭秀娟[1] 杨丽萍[1] 李颖[1] 吴乐萌[1] 王冬梅[1] 

机构地区：[1]北京大学第三医院北京大学眼科中心,100191

出　　处：《眼科研究》2010年第2期103-108,共6页Chinese Ophthalmic Research

基　　金：国家自然科学基金资助(30571986)

摘　　要：目的评价不同月龄DBA/2J小鼠的眼压、眼部特征及组织学变化。方法清洁级DBA/2J小鼠36只,3、5、7、9、11、14月龄各6只,3、9、14月龄的C57BL/6J小鼠各6只为对照。分别对实验鼠行眼前节照相,前房微管法眼压测量。用尼氏染色法对鼠视网膜切片进行染色并在光学显微镜下行视网膜神经节细胞(RGCs)计数,光学显微镜下对视网膜冰冻切片行视盘的形态学观察。结果鼠眼前节检查表明,DBA/2J小鼠从5月龄始逐渐发生虹膜色素播散、虹膜萎缩,虹膜透照可见瞳孔变形。眼压从7月龄开始升高,9月龄眼压升至高峰,14月龄降至对照组水平。各月龄DBA/2J小鼠眼压间的差异有统计学意义(F=27.600,P<0.05),各月龄C57BL/6J小鼠眼压间的差异无统计学意义(F=0.249,P=0.781)。DBA/2J鼠RGCs数量从7月龄开始减少,9～11月龄减少明显,各月龄DBA/2J鼠RGCs计数间的差异有统计学意义(F=23.594,P=0.000),各月龄C57BL/6J小鼠RGCs计数的差异无统计学意义(F=1.816,P=0.211)。DBA/2J小鼠视盘凹陷于9～14月龄开始逐渐加深,而各月龄C57BL/6J小鼠的视盘形态无明显变化。结论随月龄的增长,DBA/2J小鼠眼前节病变逐渐加重,表现出继发性青光眼的相关形态学改变。DBA/2J小鼠是研究青光眼发病机制和视神经保护较好的动物模型。Background As a hereditary chronic glaucoma model, DBA/2J mouse is widely used in the experimental research of glaucoma worldwide. Although some researches have determined the ocular change induced by hypertention of DBA/2J mouse, there is seldom reports about the research on the relationship of ocular pathological abnormality and development course in DBA/2J mouse. Objective The present study is to characterize the ocular abnormalities and histological changes induced by hypertention in different ages of DBA/2J mice. Methods The clean female DBA/2J mice aged 3-,5-,7-,9-, 11-, 14-month- old （6 mice for each） were used in this study,and age matched 18 female C57BL/6J mice were as controls. Intraocular pressure （IOP） of mice was measured by anterior chamber puncture of microneedle. The animals were sacrificed and retinal flat mounts were prepared fnr histopathological examination under the light microscope. Retinal ganglion cells （RGCs） were counted by retinal Nissl staining. Morphology of optic nerve cup in frozen section was examined under the light microscope. The experiment followed the Standard of Association for Research in Vision and Ophthalmology. Results Developing pigment dispersion, iris stroma atrophy,transillumination defects and pupil deformation were found in DBA/2J mice. IOP began to rise in 7-month-old DBA/2J mouse,peaked in 9-month-old mouse and returned to normal in 14-month-old DBA/2J mouse. A significant difference was found in IOP among different ages of DBA/2J mice （F =27.600,P 〈0.05） but not C57BL/6J mice （F =0.249,P = 0.781 ）. RGCs loss was observed in 7-month-old DBA/2J mice and more serious from 9 to ll-month-old mice, showing a significant decline of RGCs among different ages of DBA/2J mice （ F = 23. 594, P = 0. 000） but not C57BL/6J mice （ F = 1. 816, P =0. 211 ）. The abnormality of optic nerve cupping and decrease of retinal nerve fiber layer were found in 9 to 14-month-old months old DBA/2J mice and were normal in age-matched C57BL/6J mice. Conclusion The

关 键 词：DBA/2J 青光眼动物模型 高眼压 视网膜神经节细胞 

分 类 号：R775[医药卫生—眼科] R774[医药卫生—临床医学]