Adper Prompt自酸蚀粘结剂对人牙龈成纤维细胞的毒性分析  

Evaluation of cytotoxicity of Adper Prompt self-etch system dental bonding agents on human gingival fibroblasts

作　　者：何乙君[1,2] 杨小军 王琰玲[1,2] 张金艳[1,2] 雷港[1,2] 刘晨晨[1,2] 范媛[1,2] 刘卫红[1,2] 张光东[1,2] 

机构地区：[1]南京医科大学口腔医学研究所 [2]南京医科大学附属口腔医院牙体牙髓黏膜科,南京210029 [3]南京市红十字医院口腔科,南京210001

出　　处：《口腔医学》2010年第1期42-45,共4页Stomatology

摘　　要：目的分析Adper Prompt(AP)自酸蚀粘结剂对人牙龈成纤维细胞的毒性作用。方法用MTT法测定人牙龈成纤维细胞在Adper Prompt自酸蚀粘结剂不同浓度稀释液中培养4 d、7 d的吸光度(A)值,计算细胞相对增殖率(RGR),并结合流式细胞仪测定不同浓度稀释液中培养4 d后的人牙龈成纤维细胞不同增殖周期时相的DNA百分含量,计算细胞增殖指数百分比,来评估AP的细胞毒性。结果随作用时间和浓度的改变,Adper Prompt对细胞的毒性发生变化。时间越长,浓度越大,细胞相对增殖率(RGR)越小,细胞增殖指数百分比越小。结论Adper Prompt自酸蚀粘结剂在体外对人牙龈成纤维细胞有一定程度的细胞毒性,在操作过程中应采取措施保护牙龈组织。Objective To evaluate the cytotoxicity of Adper Prompt self-etch system bongding agents on human gingival fibroblasts. Methods MTT assay method was used to evaluate the cytotoxicity of Adper Prompt self-etch system bongding agents on human gingival fibroblasts. It was diluted with the culture medium by a ratio of 1 μl/L;0.5 μl/L; 0. 25 μL/L,and the fourth passage of human gingival fibroblasts were exposed to agent dilations for 4 d, 7 d respectively, and then the cytotoxicity was analyzed with MTT assay. Meanwhile, Flow Cytometry （FCM） was used to evaluate the cytotoxicity of Adper Prompt self-etch system bongding agents on human gingival fibroblasts. Results There was significant influence of Adper Prompt self-etch system bongding agent＇s exposure time and concentration on the cell morphology. Conclusions Adper Prompt self-etch system bongding agents are toxic to human gingival fibroblasts in vitro. We must use it very carefully in our practice and keep it away from gingives.

关 键 词：自酸蚀粘结剂 细胞毒性 MTT比色法 流式细胞仪 

分 类 号：R780.2[医药卫生—口腔医学]