青蒿琥酯对乙型肝炎病毒复制及肝癌细胞株HepG2.2.15凋亡的影响  

Effect of artesunate on hepatitis B virus replication and the apoptosis in the hepatoma cell line HepG2.2.15

作　　者：熊锐华[1,2] 李景怡[1] 陈贝克[3] 张荣华[1] 

机构地区：[1]第三军医大学西南医院中西医结合科,重庆400038 [2]广西桂林第181医院肿瘤科 [3]第三军医大学学员旅12队

出　　处：《胃肠病学和肝病学杂志》2010年第1期71-74,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨青蒿琥酯在体外对乙型肝炎病毒复制及肝癌细胞株HepG2.2.15凋亡的影响。方法将不同浓度青蒿琥酯作用于转染乙型肝炎病毒全基因组DNA的肝癌细胞株HepG2.2.15,收集48 h上清,采用酶联免疫吸附实验检测上清中乙型肝炎病毒表面抗原(HBsAg)和e抗原(HBeAg),采用荧光定量PCR法检测HBV-DNA,流式细胞术检测细胞凋亡。结果青蒿琥酯对HBV复制具有抑制作用,随着浓度增加,对HBsAg和HBeAg的抑制率逐渐上升,细胞内HBV-DNA复制水平下降;青蒿琥酯可诱导肝癌细胞早期凋亡及导致细胞死亡,随浓度增加,HepG2.2.15细胞早期凋亡率及死亡率均增加。结论青蒿琥酯对HepG2.2.15细胞HBsAg和HBeAg的分泌及HBV-DNA复制具有抑制作用,并具有诱导HepG2.2.15细胞凋亡的作用。Objective To investigate the effect of artesunate on hepatitis B virus（HBV） replication and the apoptosis in the hepatoma cell line HepG2.2.15.Methods The HepG2.2.15 cells were treated with different concentrations of artesunate for 48 hours.Hepatitis B surface antigen（HBsAg） and hepatitis B e antigen （HBeAg） in supernatant were detected by ELISA,HBV-DNA was decteted by fluorescence quantitative PCR,and HepG2.2.15 cell apoptosis was detected by Flow cytometry.Results Artesunate suppressed expressions of HBsAg and HBeAg,and inhibited the replication of HBV-DNA.Artesunate induced HepG2.2.15 cell early apoptosis and leaded to HepG2.2.15 cell death.With the artesunate concentration higher,the role was stronger.Conclusion Artesunate suppresses expressions of HBsAg,HBeAg and replication of HBV-DNA in HepG2.2.15 cells,and induces HepG2.2.15 cell apoptosis.Artesunate could be used to treat posthepatitic primary hepatic carcinoma.

关 键 词：青蒿琥酯 乙型肝炎 原发性肝癌 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]