超滤技术去除苦参素注射液的细菌内毒素  

Elimination of Bacterial Endotoxins in Kushensu Injection by Ultrafiltration Device

作　　者：宫凯敏[1] 李红阳[1] 尹楠[1] 彭国平[1] 李存玉[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《辽宁中医药大学学报》2010年第2期18-19,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家科技支撑计划课题(2006BAI06A04)

摘　　要：目的:考察超滤技术去除苦参素注射液中细菌内毒素的效果。方法:采用10KDa截留分子量超滤膜对苦参素注射液进行超滤,以动态浊度法定量检测超滤前后苦参素注射液中细菌内毒素的含量。结果:样液超滤前细菌内毒素为8.0540EU/mL,超滤后为0.3024EU/mL,内毒素去除率为96.24%,其有效成分氧化苦参碱的透过率为96%。结论:超滤技术可以很好的去除苦参素注射液中细菌内毒素,有效成分损失少,可提高其用药安全性。Objective：To studies the elimination effect of bacterial endotoxins in Kushensu injection by ultrafiltration device. Method：Kushensu injection was ultrafiltrated with molecular weight cut-off （MWCO） 10k ultrafitration membrane. The kinetic-turbidimetric method was used to determine the content of bacterial endotoxins in Kushensu injection before and after using the ultrafiltration. Result：The content of bacterial endotoxins was 8.0540EU/mL before ultrafitration,and 0.3024EU/mL after ultrafitration.The elimination efficiency of bacterial endotoxins was 96.24%,the transmittance of effective constituent of Oxymatrine was 96%. Conclusion：Bacterial endotoxins in Kushensu injection can be well eliminated by ultrafiltration,the effective constituent was little missed,and the safety of drug could be improved.

关 键 词：超滤 苦参素注射液 细菌内毒素 

分 类 号：R2-03[医药卫生—中医学]