谷氨酸引起培养大鼠星形胶质细胞水通道蛋白4表达动态变化  

Glutamate-induced Dynamic Expression of Aquaporin 4 in Cultured Rat Astrocytes

作　　者：路杨[1] 师忠芳[1] 袁芳[1] 韩明[1] 

机构地区：[1]首都医科大学北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2010年第1期29-31,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(30840083);北京市自然科学基金资助项目(7062012)

摘　　要：目的研究谷氨酸是否引起培养大鼠星形胶质细胞水通道蛋白4(AQP4)表达变化。方法利用传代培养至10d左右的大鼠星形胶质细胞,给予1mmol/LL-谷氨酸钠,分别作用1h、3h、6h、12h、24h、48h后进行细胞形态学观察、GFAP免疫化学染色及荧光定量PCR检测AQP4mRNA表达变化。结果谷氨酸作用1h后星形胶质细胞出现肿胀并随着作用时间延长肿胀持续,AQP4mRNA3h时开始出现表达降低(P<0.05),后随谷氨酸作用时间延长先下调后上调,12h达最低(P<0.01),48h升至高于正常(P<0.05)。结论AQP4可能在谷氨酸引起星形胶质细胞肿胀发生发展中发挥作用。Objective To determine whether glutamate induces the alteration of aquaporin 4 （AQP4） expression in cultured rat astrocytes. Methods The secondary cultured astrocytes were treated with 1 mmol/L L-glutamate for 1 h, 3 h, 6 h, 12 h, 24 h and 48 h. The morphologic changes of astrocytes were observed through microscope after GFAP immunostaining and AQP4 mRNA ex pression were detected with real-time PCR. Results The astrocytes swelled when exposed to glutamate for 1 h and remained with prolonged treatment. Meanwhile, the AQP4 mRNA expression were early down-regulated and subsequently up-regulated, featured with the lowest AQP4 mRNA level at 12 h after treatment （P〈0.01） and higher at 48 h （P〈0.05）. Conclusion Aquaporin 4 may be involved in the occurrence and development of astrocyte swelling induced by glutamate.

关 键 词：谷氨酸 水通道蛋白4 星形胶质细胞 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]