三氧化二砷对人肝癌细胞生长增殖的影响  

EffectS of Arsenic Trioxide on Growth and Proliferation of Human Hepatoma Cancer Cells in Vitro

作　　者：张霞[1] 

机构地区：[1]山东省肿瘤医院外科,250117

出　　处：《实用癌症杂志》2010年第1期23-25,30,共4页The Practical Journal of Cancer

摘　　要：目的观察三氧化二砷(As2O3)对人BEL7402肝癌细胞株的作用及其机制。方法采用MTT法检测As2O3对BEL7402细胞的生长抑制率;流式细胞仪测定经不同浓度As2O3处理后的BEL7402细胞周期及增殖细胞核抗原(PCNA)变化,同时Annexin V-FITC/PI双染法检测细胞凋亡。结果三氧化二砷可显著抑制BEL7402细胞生长,且剂量-效应关系显著(γ=0.9650,P<0.01),半数抑制浓度(IC50)为4.93μmol/L;BEL7402经As2O3处理后可发生凋亡。As2O3能显著下调PCNA蛋白表达(P<0.01),并使细胞周期阻滞在G2/M期。结论三氧化二砷可有效抑制人肝癌BEL7402细胞生长增殖,其机制可能与诱导细胞凋亡、下调PCNA表达及阻滞细胞周期有关。Objective This study was to research the effeet of arsenie trioxide （ As2O3 ） on human hepatoma cancer cell line BEE7402 and its meehanisms in vitro. Methods MTT assay was used to observe the growth inhibitory rate of BEL7402 cells treated with As2O3 ;the eell eyele,apoptosis and proliferation cell nuclear antigen （PCNA） changes were measured by flow eytometry in BEL7402 eells treated with As2O3. Results As2O3 eould inhibit the growth of BEL7402 cells dramatically. There was obvious dosage-effeet correlation （γ= 0. 965 0 ,P 〈 0.01 ）, its half inhibitory concentration （IC50） was 4.93 μmol/L. Apoptosis was observed in BEL7402 cells treated with As2O3. As2O3 could deerease PCNA protein expression and induce eell cycle arrest in G2/ M phase （P 〈 0.01 ）. Conclusion As2O3 eould inhibit the growth and proliferation of BEL7402 cells in vitro dramatically, its mechanisms were probably associated with inducing apoptosis ,decreasing PCNA expression and cell cycle arrest.

关 键 词：三氧化二砷 肝癌细胞株 增殖细胞核抗原 细胞周期 流式细胞术 

分 类 号：R735.7[医药卫生—肿瘤]