Survivin、caspase-3及AKT1在大肠癌中的表达及意义  

The Expression and Significance of survivin,caspase-3 and AKT1 in Colorectal Adenocarcinoma

作　　者：樊勇军[1] 冯一中[2,3] 李海[4] 郑晓娟[1] 

机构地区：[1]昆山市第一人民医院普外科,215300 [2]苏州大学病理教研室 [3]苏州大学附属第二人民医院病理科,215123 [4]昆山市第一人民医院病理科,215300

出　　处：《实用癌症杂志》2010年第1期26-30,共5页The Practical Journal of Cancer

摘　　要：目的探讨survivin、caspase-3和AKT1在大肠腺癌中的表达及其临床病理学意义以及三者在大肠腺癌癌变过程中的作用。方法运用免疫组织化学SP法检测58例大肠腺癌、12例大肠腺瘤和10例正常大肠黏膜中survivin、caspase-3和AKT1的表达情况,并分析其与大肠腺癌临床病理特征之间的关系以及三者之间的关系。结果在大肠腺癌中survivin、caspase-3以及AKT1的阳性表达率分别为77.6%(45/58)、32.8%(19/58)及70.7%(41/58)。Survivin在大肠腺癌中的表达高于大肠腺瘤以及正常大肠黏膜(P<0.05,P<0.05),cspase-3在大肠腺癌中的表达低于正常大肠黏膜(P<0.05),AKT1在大肠腺癌中的表达高于正常大肠黏膜(P<0.05)。Survivin的表达与肿瘤分化程度关系密切(P<0.05),caspase-3的表达与肿瘤分化程度、淋巴结转移关系密切(P<0.05,P<0.05),AKT1的表达与TNM分期关系密切(P<0.05)。Survivin的表达与caspase-3的表达呈显著负相关(P<0.05),与AKT1的表达呈显著正相关(P<0.05)。结论Survivin与AKT1在大肠腺癌中高表达,caspase-3在大肠腺癌中低表达,且survivin与caspase-3的表达显著负相关,survivin与AKT1的表达显著正相关,提示survivin可能下调caspase-3的表达而上调AKT1的表达,共同促进大肠腺癌的发生发展。Objective To detect the expression and its clinicopathologic effects of survivin,caspase-3 and AKT1 protein in colorectal adenocarcinoma,and to explore the roles of the three factors in the development of colorectal adenocarcinoma. Methods Immunohistochemical SP staining method was used to detect the expression of s urvivin, caspase-3 and AKT1 in 58 cases of colorectal adenocarcinoma, 12 cases of colorectal adenoma and 10 cases of normal colorectal mucosa. And we analyze the relation- ship between these factors and clinicopathologic characteristics,and the relationship the three factors. Results The positive rates of survivin,caspase-3 and AKTI were 77.6% （45/58） ,32.8% （19/58） and 70.7% （41/58） in colorectal adenocarcinoma,respectively. The positive rates of survivin in colorectal adenocarcinoma were significantly higher than colorectal adenoma and normal colorectal mucosa （ P 〈 0.05, P 〈 0.05 ）. The positive rates of caspase-3 in colorectal adenocarcinoma were significantly lower than normal colorectal mucosa （ P 〈 0.05 ）. The positive rates of AKT1 in colorectal adenocarcinoma were significantly higher than normal colorectal mucosa （ P 〈 0.05 ）. The expression of survivin in 58 cases of colorectal adenocarcinoma was significantly correlated with the degree of differentiation（ P 〈 0.05 ）. The expression of caspase-3 in 58 cases of colorectal adenocarcinoma was significantly correlated with the degree of differentiation and the lymph node metastasis （ P 〈 0.05, P 〈 0.05 ）. The expression of AKT1 in 58 cases of colorectal adenocarcinoma was significantly correlated with TNM staging（ P 〈 0.05 ）. Spearman correlation showed that there was a significant negative correlation between survivin and caspase-3 （ P 〈 0.05 ）, a significant positive correlation between survivin and AKT1 （ P 〈 0.05 ）. Conclusion High positive expression of survivin and AKTl,low positive expression of caspase-3 in colorectal adenocarcinoma, and significant negative correlation bet

关 键 词：大肠腺癌 SURVIVIN基因 CASPASE-3基因 AKT1基因 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]