胃癌组织中T细胞及其亚群ICOS分子的表达及其意义  

Expression of Inducible Costimulator and Variance of T Cell Subpopulations in Patients with Gastric Cancer

作　　者：李天威[1] 许国岩 赵国华[1] 

机构地区：[1]辽宁省肿瘤医院,110042 [2]辽宁陆军预备役高射炮兵一师三团卫生队

出　　处：《实用癌症杂志》2010年第1期34-36,共3页The Practical Journal of Cancer

基　　金：辽宁省科技厅博士启动基金资助项目(20071028)

摘　　要：目的探讨胃癌组织中T细胞共刺激分子ICOS及其亚群的表达及意义。方法应用流式细胞术检测38例胃癌和21例健康人(对照组)外周血T细胞亚群及其共刺激分子ICOS的表达。结果胃癌组与对照组比较:CD3+T细胞表达(53.61±13.84)/(72.07±7.83)%,P<0.01;CD3+CD4+T细胞表达(29.84±9.71)/(38.79±5.08)%,P<0.01;CD3+ICOS+T细胞表达(25.80±10.56)/(0.82±0.98)%,P<0.01;CD3+CD8+ICOS+T细胞表达(1.57±1.99)/(0.02±0.04)%,P<0.01;CD3+CD8+ICOS-T细胞表达(16.06±6.94)/(20.56±6.54)%,P<0.05。胃癌组患者手术前和手术后1周外周血T细胞亚群的差异无统计学意义(P>0.05)。结论胃癌患者T细胞数量明显减少,T细胞共刺激分子ICOS表达增高,CD4+T细胞显著减少。Objective To investigate the role of expression of T cell costimulatory molecule ICOS and variance of T cell subpopulations in the development and prognosis of gastric cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule ICOS by flow cytometry in 38 patients with gastric cancer and 21 healthy peoples as control group. Results The expressions of T cell costimulatory molecule ICOS in the gastric cancer and healthy controls were （25.80 ± 10.56） % and （0.82 ±0.98） % （P 〈0.01 ） ; expression percentage of total T cell （ CD3^＋ ） were （53.61 ±13.84）vs（72.07 ±7.83）% ,P 〈0.01 ; expression percentage of CD4^＋T cell （CD4^＋ CD3^＋） were （29.84 ±9.71）vs （38.79 ± 5.08 ） %, P 〈 0.01 ; expression percentage of CTL cell （ CD8^＋ ICOS ^＋ CD3 ^＋ ） were （ 1.57 ± 1.99 ） vs （ 0.02 ± 0.04 ） %, P 〈 0.01 ; expression percentage of CD8^ ＋ inhibitory T cell （ CD8^＋ICOS ^- CD3 ^＋ ） ratio were （ 16.06 ± 6.94） vs （ 20.56 ± 6.54 ） %, P 〈 0.05. No difference of expression was found in peripheral T cell subpopulations of pestoperativc patients with gastric cancer after one week （ P 〉 0.05 ）. Conclusion T cell number is fall, and T cell eostimulatory molecule ICOS useless expression is increase in patients with gastric cancer. CD4^＋ ＋T cell subpopulation is significantly decreased in patients with gastric cancer.

关 键 词：胃癌 ICOS分子 T细胞亚群 

分 类 号：R735.2[医药卫生—肿瘤]