枸杞多糖对染铅小鼠学习记忆及海马区PKC影响  

Influenec of Lycium barbarum polyccharides on learning and menory function and protein kinase C in hippocampus in lead-exposed mice

作　　者：蔡天革[1] 蔡宇[2] 余绍蕾[2] 

机构地区：[1]辽宁大学生命科学学院,沈阳110036 [2]暨南大学药学院

出　　处：《中国公共卫生》2010年第1期38-39,共2页Chinese Journal of Public Health

基　　金：广东省科技规划项目(2008B011000004)

摘　　要：目的探讨枸杞多糖对染铅小鼠学习记忆功能和海马区蛋白激酶C(Protein kinase C,PKC)的影响。方法将实验小鼠随机分为5组,即空白对照组、染铅模型组、枸杞多糖高、中、低3个剂量组,枸杞多糖各剂量组于染铅后第2d灌胃给药,每天1次,于第2,4周测小鼠到达迷宫避难台所需要的时间,第4周测血铅浓度和海马区PKC含量。结果枸杞多糖各剂量组与染铅模型组比较,血铅浓度[枸杞多糖各剂量组(0.65±0.28)～(0.11±0.13)mg/L,模型组(1.68±0.19)mg/L]明显降低,到达避难台的时间也明显减少;而海马区PKC含量[枸杞多糖各剂量组(73.23±6.52)～(96.33±8.03)mmol/L,模型组(62.26±7.21)mmol/L]明显升高,差异有统计学意义(P<0.05或P<0.01)。结论枸杞多糖可促进小鼠体内铅的排出,改善染铅小鼠的学习记忆功能,抑制海马区PKC含量降低。Objective To explore the influence of Lycium barbarum polyccharides（LBP）on learning and memory function and the content of protein kinase C（PKC）in hippocampus of lead-exposed mice.Methods The mice were divided randomly into five groups：control group,lead-exposed model group,high-,mid-and low-dosage LBP groups.The LBP groups were fed with different dosage of LBP since the second day of the lead exposure.Blood-lead concentration,time of reaching water maze platform at 2nd and 4th week,and the concentration of PKC in hippocampus wrere detected.Results Compared with lead-exposure model group,the blood-lead concentrations of the mice in LBP groups was lower（0.65±0.28-0.11±0.13 mg/L vs 0.68±0.19 mg/L） and the time of reaching at the platform was less.The concentration of PKC in LBP groups（73.23±6.52-96.33±8.03 mmol/L） was higher than that of in lead-explsed model guoup（62.26±7.21 mmol/L）.All the diffenrences were statistically significant （P0.05）.Conclusion LBP promotes the discharge of lead and improves learning and memory function,and inhibits PKC decrease in hippocampus in lead-exposed mice.

关 键 词：枸杞多糖 铅 海马区 蛋白激酶C 

分 类 号：R151.3[医药卫生—营养与食品卫生学] R995[医药卫生—公共卫生与预防医学]