生长期缺锌对大鼠海马Egr家族基因表达影响  

Effect of zinc deficiency on expression of Egr gene in hippocampus of growing rats

作　　者：余晓丹[1] 颜崇淮[1] 余晓刚 沈晓明[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所上海市环境与儿童健康重点实验室,上海200092

出　　处：《中国公共卫生》2010年第1期57-59,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30600496);上海市重点实验室(06DZ22024);上海市儿科学重点学科(T0204);上海市医学重点学科(05Ⅲ002)资助项目

摘　　要：目的研究锌缺乏对生长期大鼠海马Egr家族(Egr1、Egr2、Egr3、Egr4)基因表达的影响,探讨缺锌影响学习记忆的基因转录调节机制。方法选取刚出生的Sprague-Dawley大鼠3窝,每窝1只母鼠、10只仔鼠,3窝母鼠与仔鼠体重相近,仔鼠雌雄各半;随机分为3组:缺锌组(ZD)、配饲组(PF)、对照组(ZA),实验期60d;用实时荧光定量PCR检测大鼠海马Egr家族(Egr1、Egr2、Egr3、Egr4)mRNA的表达。结果大鼠海马Egr1、Egr3、Egr4基因表达在3组大鼠间差异有统计学意义(F值分别为13.631,24.435,21.825,),ZD组(2.44×10-3,8.72×10-3,1.88×10-2)表达量低于PF组(3.13×10-3,1.60×10-2,2.68×10-2)、ZA组(3.96×10-3,1.92×10-2,3.53×10-2),且PF组低于ZA组;海马Egr2基因表达在各组间差异无统计学意义。结论缺锌使海马锌指蛋白Egr1、Egr3、Egr4基因表达下调,是影响海马学习记忆的机制之一。Objective To study the effect of zinc deficiency on the expression of Egr gene in hippocampus of rats.Methods Thirty new born SD rats and their mothers were randomly divided into three groups：zinc adequation（ZA）,zinc deficiency（ZD）,paired-fed（PF）.After 60 days feeding,the RNA in hippocampus was extracted and the levels of Egr1,Egr2,Egr3,Egr4 mRNA were measured with real time fluorescent quantity PCR.Results The Egr1,Egr3,Egr4 gene expressions in hippocampus among the three groups were different significantly（F=13.631,24.435,21.825,P0.05）.The Egr1,Egr3,Egr4 gene expressions in hippocampus of ZD group were lower than that of PF group.While the Egr1,Egr3,Egr4 gene expressions in hippocampus of PF group were lower than that of ZA group.However,there was no difference of the Egr2 expression in hippocampus among three groups.Conclusion Downregulation of Egr1,Egr3,Egr4 mRNA expression in hippocampus by zinc deficiency is one of the mechanism of zinc deficiency affecting the learning and memory.

关 键 词：锌缺乏 Egr基因 基因表达 

分 类 号：R153.2[医药卫生—营养与食品卫生学]