Midkine特异的shRNA对胃肿瘤细胞BGC823增殖及凋亡的影响  

The effect of the specific shRNA of midkine on the proliferation and apoptosis of gastric carcinoma cell BGC823

作　　者：王庆苓[1] 黄亚红[2] 柳红[1] 侯亚义[2] 

机构地区：[1]徐州医学院病理学教研室,江苏徐州221004 [2]南京大学医学院免疫与生殖生物学实验室和国家生物医药技术重点实验室,江苏南京210093

出　　处：《徐州医学院学报》2010年第1期23-27,共5页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30872941);徐州医学院院长人才专项基金(09KJZ12)

摘　　要：目的研究shRNA干扰midkine对胃肿瘤细胞BGC823增殖能力及凋亡的影响。方法构建midkine基因的特异性小RNA干扰质粒,采用脂质体2000转染BGC823细胞,运用CCK-8检测细胞的增殖能力,PI染色检测细胞凋亡情况。结果成功转染重组体的BGC823细胞增殖明显受到抑制(P<0.01)细胞形成克隆数明显降低(P<0.01),并且有明显的亚二倍体峰出现(P<0.05)。结论针对midkine基因的特异性小RNA干扰质粒在体外能有效抑制人胃癌细胞BGC823 midkine表达,细胞增殖能力减弱,细胞凋亡增加,为midkine基因靶向治疗提供一定的实验依据。Objective To investigate the effect of knockdown of midkine（MK） expression by shRNA on cell proliferation and apoptosis of human gastric carcinoma cell BGC823.Methods Specific shRNA plasmids to midkine were constructed,and were then transfected into BGC823 by lipofectamin 2000.The cell proliferation was evaluated by CCK-8 kit,and the apoptosis was determined by flow cytometric analysis.Results The recombinant plasmid expressing midkine shRNA effectively inhibited BGC823 cell proliferation（P〈0.01） and decreased clone formation（P〈0.01） and significantly promoted cell apoptosis with the emergence of sub-diploid peak（P〈0.05）.Conclusion shRNA plasmids targeting MK gene can inhibit the growth of human gastric cancer cells by attenuating cell proliferation and inducing apoptosis,which might provide experimental evidence for gene targeting therapy of MK.

关 键 词：MIDKINE SHRNA 胃肿瘤细胞 细胞增殖 细胞凋亡 

分 类 号：R73-3[医药卫生—肿瘤]