亚砷酸氧化菌Sinorhizobium sp. GW3的鉴定与亚砷酸氧化酶基因的分离  

Identification of A Novel Arsenite-Oxidizing Bacterium Sinorhizobium sp. GW3 and Isolation of Arsenite Oxidase Gene

作　　者：汪耀[1] 涂书新[2] 王革娇[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室/生命科学技术学院,武汉430070 [2]华中农业大学资源与环境学院,武汉430070

出　　处：《湖北农业科学》2010年第1期5-9,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30671140);教育部博士点基金项目(20060504027)

摘　　要：亚砷酸氧化细菌能够将毒性大的As(Ⅲ)氧化成毒性小的As(Ⅴ),在生物修复砷污染方面具有应用价值。对一株新型亚砷酸氧化菌Sinorhizobium sp.GW3进行了较全面的鉴定,并分离及分析了亚砷酸氧化酶基因aoxAB。形态学、生理生化鉴定和16S rRNA基因等分析结果表明该菌为Sinorhizobium属。该菌对As(III)抗性的MIC(Minimum inhibitory concentration)为9 mmol/L。首次在Sinorhizobium属中分离了包括编码小亚基aoxA和大亚基aoxB在内的亚砷酸氧化酶基因,其编码的大小亚基与已发现的Agrobacterium tumefaciens(ABB51929)的大小亚基在氨基酸水平上分别有86%、80%的同源性。Abstract： In this study, a novel arsenite-oxidizing bacterium GW3 was identified and its arsenite oxidase gene aoxAB was isolated. Based on the morphological, physiological/biochemical characteristics and 16S rRNA gene sequence analyses, this strain was identified as Sinorhizobium sp. GW3. The MIC （Minimum Inhibitory Concentration） for arsenite was 9 mmol/L. This was the first report to isolate full length arsenite oxidase gene including aoxA and aoxB from Sinorhizobium. The aoxA and aoxB of Sinorhizobium sp. GW3 showed 80% and 86% amino acid identities with the aoxA and aoxB of Agrobacterium tumefaciens （ABBS1929）, respectively.

关 键 词：砷 亚砷酸氧化菌 亚砷酸氧化酶基因 反向PCR SINORHIZOBIUM 

分 类 号：Q939.99[生物学—微生物学] Q789