道地药材五鹤续断的染色体制片优化与核型分析  

Optimization of Chromosomes Sectioning and Karyotpe Analysis of Radix Dipsacus

作　　者：施波[1] 雷盼[1] 石开明[1] 周毅峰[1] 周光来[1] 丁莉[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《湖北农业科学》2010年第1期129-131,共3页Hubei Agricultural Sciences

基　　金：国家民委项目(08HB06);生物资源保护与利用湖北省重点实验室(湖北民族学院)资助项目(2007025)

摘　　要：以恩施地区五鹤续断的根尖为材料,研究了预处理时间和解离时间对其染色体制片的影响,并用此最佳条件进行了五鹤续断根尖染色体核型分析。结果表明,0.1%秋水仙素预处理2.5h、1mol/L盐酸解离30min的处理条件为最佳;五鹤续断染色体数目为18条,核型公式为2n=18=12m+4sm+2st,核型为2A型。The roots of Wu-He Dipsacus in Enshi were used to study the effect of pre-treating time and the time of dissociation on the slide-preparing of the chromosomes.The results revealed that the pretreatment with 0.1% eolchicines 2.5h and lmol/L HCl at 60 temperature degree 30min preformed well in the sectioning. This technique was employed to determine the karyotype of Wu-He Dipsacus. The results showed that the number of chromosomes in Wu-He Dipsacus was 18 and the formula was 2n=18=12m＋4sm＋2st, and karyotype asymmetry belonged to 2A type.

关 键 词：五鹤续断 染色体制片 核型 

分 类 号：S567.23[农业科学—中草药栽培]