转基因杨树外源基因PCR检测模板DNA的快速制备  

A rapid preparation of template DNA for PCR detection of extraneous genes of transgenic Populus tomentosa

作　　者：王晓锋[1,2] 潘建芝 李燕玲[1] 杜克久[1] 

机构地区：[1]河北农业大学,河北保定071000 [2]南京林业大学,江苏南京210037 [3]晋冀鲁豫烈士陵园管理处,河北邯郸056001

出　　处：《河北林果研究》2009年第4期349-352,共4页Hebei Journal of Forestry and Orchard Research

基　　金：河北省科技厅资助项目(03547016D);河北省林业局资助项目(0207216)

摘　　要：用带盖离心管定量取转基因毛白杨组培苗及其室外8 a生植株叶片,加入DNA提取缓冲液研磨,离心后取上清液稀释5倍。取1μL作为PCR模板DNA检测杨树外源Bt和Npt II基因。PCR产物经琼脂糖凝胶电泳检测,目的条带清晰,可重复性强。The leaves of tissue culture stocks and of 8 years old trees of transgenie Populus tomentosa were taken as materials using centrifuge tube. The materials were grinded for 15 - 20 s with DNA extraction buffer at room temperature. Samples were centrifuged,and the supematants of 5- fold diluted were used as a PCR template DNA to detect the Bt gene and the Npt II gene.

关 键 词：转基因毛白杨 模板DNA制备 PCR检测 

分 类 号：S792.11[农业科学—林木遗传育种]