人卵巢癌微血管内皮细胞的培养及体外管腔样结构形成的观察  

Incubation of human ovarian cancer microvascular endothelial cells and formation of lumen-like structure in vitro

作　　者：徐燕[1] 王丹[1] 谢尧[1] 梁志清[1] 

机构地区：[1]第三军医大学西南医院妇产科,重庆400038

出　　处：《第三军医大学学报》2010年第3期201-205,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金(30600673);全军医学科研"十一五"计划青年基金(06Q054)~~

摘　　要：目的建立人卵巢癌微血管内皮细胞(ovarian carcinoma-derived microvascular endothelial cells,ODMECs)免疫磁珠分选体系并观察ODMECs体外二维管腔样结构(tubule-like structure,TLS)的形成。方法利用结合有CD31抗体的MACS MicroBeads免疫磁珠分选系统分离纯化ODMECs。通过形态学、生化和表型特征对所获得的ODMECs进行鉴定;并检测其摄取乙酰化低密度脂蛋白(acetylated-low-density lipoprotein,DIL-acLDL)和结合凝集素Ⅰ(UEA-Ⅰ)的能力,观察其在体外培养体系中二维管腔样结构的形成。结果所获ODMECs流式分析细胞纯度可达99.6%,具有卵石征、接触抑制,表达CD31、CD105、FⅧ-Rag;具有结合UEA-Ⅰ和摄取DIL-acLDL的能力;培养过程中观测到ODMECs可形成二维管腔样结构。结论成功建立了人ODMECs的免疫磁珠分离培养方法,并观察到ODMECs具有体外二维管腔样结构。Objective To establish the magnetic activated＇ cell sorting system （MACS） for isolation and incubation of human ovarian carcinoma-derived microvascular endothelial cells （ODMECs） and to observe their ability to form tubule-like structure （TLS） in vitro. Methods MACS was used to purify ODMECs with antiCD31 antibody and ODMECs were identified according to their morphological, biochemical and phenotypic characteristics. The ability of ODMECs to intake acetylated-low-density lipoprotein （DIL-acLDL） and Ulex europeans agglutinin-1 （ UEA- Ⅰ ） was assayed to observe the formation of TLS in vitro. Results Flow cytometry showed that the purity of ODMECs was up to 99.6%. The ODMECs were characterized by cobble stones, contact inhibition, expressed CD31,CD105 and FⅧ-Rag, and exhibited the ability to bind to UEA-1,intake DIL-acLDL and form TLS during incubation. Conclusion The MACS we established can be used to isolate and incubate human ODMECs and study the formation characteristics of TLS.

关 键 词：卵巢肿瘤 毛细血管 内皮细胞 细胞 培养的 免疫磁化分离 

分 类 号：R737.31[医药卫生—肿瘤] R322.13[医药卫生—临床医学]