模拟高原缺氧对慢性牙周炎大鼠血清中MMP-2表达的影响  

Effect of simulated high altitude hypoxia on matrix metalloproteinase-2 expression in serum of rats with chronic periodontitis

作　　者：孔耀[1] 刘鲁川[1] 周霞[1] 刘福玉[2] 杨苏平[1] 张海元[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所口腔科,重庆400042 [2]三军医大学高原军事医学系病理生理学与高原生理学教研室,高原医学教育部重点实验室,全军高原生理与高原病研究重点实验室,重庆400038

出　　处：《第三军医大学学报》2010年第4期324-326,共3页Journal of Third Military Medical University

基　　金：全军医学科研“十一五”计划面上项目(06MB252)~~

摘　　要：目的研究常氧与缺氧条件下慢性牙周炎大鼠血清中基质金属蛋白酶2(matrixmetalloproteinase-2,MMP-2)的表达变化,探讨MMP-2与高原牙周病的关系。方法模拟高原缺氧建立大鼠慢性牙周炎模型,采用ELISA法测定正常对照组、常氧牙周炎组、缺氧对照组、缺氧牙周炎组大鼠血清中MMP-2表达。结果正常对照组、常氧牙周炎组、缺氧对照组、缺氧牙周炎组血清中MMP-2浓度分别为(92.00±21.78)、(118.09±25.01)、(102.03±21.30)、(159.19±34.13)ng/ml,常氧牙周炎组与正常对照组、缺氧牙周炎组与缺氧对照组、缺氧对照组与正常对照组、缺氧牙周炎组与常氧牙周炎组比较均有统计学意义(P<0.01)。其中缺氧牙周炎组血清中MMP-2浓度升高最为显著(P<0.01)。结论缺氧上调血清中MMP-2的表达,进而促进大鼠牙周组织的破坏。Objective To study the change of matrix metalloproteinase-2 （MMP-2） expression in serum of rats with chronic periodontitis under normal oxygen or hypoxia conditions and the correlation between MMP-2 and high altitude periodontitis. Methods A chronic periodontitis model of rat was established by simulating high altitude hypoxia. Rats were divided into normal control （NC） group, normal oxygen periodontitis group （P1）, hypoxia control group（ H）, and hypoxia periodontitis group （P2）. Expression of MMP-2 in serum of rats was detected by ELISA. Results The MMP-2 level in N, P1, H, and P2 groups was 92.00 ±21.78, 118.09 ± 25.01, 102.03 ± 21.30, and 159.19 ± 34.13 ng/ml, respectively （P 〈 0. 01 ）. The serum MMP-2 level was significantly higher in P2 group than in other groups. Conclusion Hypoxia up-regulates MMP 2 expression in serum and promotes destruction of periodontal tissue in rats, suggesting that MMP-2 participates in destruction of periodontal tissue during hypoxia periodontitis, which may be related with the recurrence and development of high altitude periodontal diseases.

关 键 词：缺氧 基质金属蛋白酶2 血清 牙周炎 大鼠 

分 类 号：R339.54[医药卫生—人体生理学] R446.112[医药卫生—基础医学]