nNav1.5在人脑星形细胞瘤中的表达及意义  

Expression and clinical significance of nNav1.5 in human astrocytomas

作　　者：欧绍武[1] 王军[1] 王运杰[1] 宗志红[2] 

机构地区：[1]中国医科大学附属一院神经外科,辽宁沈阳110001 [2]中国医科大学生物化学与分子生物学教研室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2010年第2期241-244,共4页Journal of Modern Oncology

基　　金：辽宁省自然科学基金(编号:20082110);辽宁省教育厅资助项目(编号:05L500)

摘　　要：目的:研究nNav1.5 mRNA及蛋白在人脑星形细胞瘤组织和正常脑组织中的表达差异。方法:采用逆转录聚合酶链反应(RT-PCR)及western blot法分别检测nNav1.5 mRNA及蛋白在30例人脑星形细胞瘤组织和8例正常脑组织中的表达水平。结果:人脑星形细胞瘤组织中nNav1.5基因及蛋白的表达值显著高于正常脑组织(P<0.01),且随病理级别的增高而升高。结论:nNav1.5 mRNA及蛋白在人脑星形细胞瘤组织中的表达水平显著升高,这可能与星形细胞瘤细胞增殖活跃及侵袭性生长等生物特性有关。Objective：To explore the expression difference of nNav1. 5 in human astroeytomas and normal brain tissue. Methods： Polymerase ehain reaction with reverse transcrpfion （ RT - PCR） and western blot methods were performed to detect the expression level of nNavl. 5 mRNA and protein. Results ： nNavl. 5 mRNA and protein could be detected in 30 human astroeytoma specimens and 8 normal brain tissues, but their expression values in astrocytomas were considerably higher than those in normal brain tissues （ P 〈 0.01 ）. And the expression values of nNavl. 5 increased with the increasing of pathological grades. Conclusion： The expression level of nNavl. 5 in astroeytomas were considerably higher than those in normal brain tissues ,whieh may be related to glioma cell proliferation and invasion.

关 键 词：nNav1.5 星形细胞瘤 基因表达 Na+通道 MRNA 蛋白 

分 类 号：R730[医药卫生—肿瘤] R739.4[医药卫生—临床医学]